| Applicable For |
All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
| Elution Volume |
= 37.5 µl DNA Elution Buffer or DNase free water |
| Equipment |
Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block |
| Processing Volume |
Fecal =100 mg, Soil = 200 mg, Cells = 20 mg (Approximately equal to 2×108 bacterial, 2×107 yeast, or 2×106 mammalian cells) |
| Purity |
High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing |
| Sample Source |
Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
| Sample Storage |
Eluted DNA should be stored at = -20°C. |
| Size Range |
Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
| Type |
Total DNA |
| Yield |
Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum) |
