Applicable For |
All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
Elution Volume |
= 20 µl DNA Elution Buffer or DNase free water |
Equipment |
Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer |
Processing Volume |
Fecal =100 mg, Soil = 100 mg, Cells = 20 mg (Approximately equal to 2×108 bacterial, 2×107 yeast, or 2×106 mammalian cells) |
Purity |
High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing |
Sample Source |
Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
Sample Storage |
Eluted DNA should be stored at = -20°C. |
Size Range |
Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
Type |
Total DNA |
Yield |
Up to 5 µg total DNA can be eluted into 20 µl |