- Simple: Fast spin-column procedure yields pure yeast RNA without using glass beads or phenol.
- Versatile: Efficient RNA isolation from a broad spectrum of fungal species susceptible to Zymolyase.
- High-Quality: Isolated RNA is suitable for use in RT-PCR, Northern blotting, etc.
The YeaStar RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans/, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 µg of total RNA from 1-1.5 ml of cultured cells using innovative Zymo-Spin column technology.
|Applicable For||Isolated RNA is well suited for downstream applications such as Northern blotting, RT-PCR, Next-Generation Sequencing, Microarray, hybridization and other sensitive applications|
|Elution Volume||= 60 µl DNase/RNase-Free Water|
|Equipment||Microcentrifuge & Heat Block/Bath|
|Processing Time||30 minutes|
|Processing Volume||= 1.5 ml of Culture (1-5 x 107 cells)|
|Purity||Typical A260/A280 & A260/A230 = 1.8|
|Sample Source||Cell Culture (Colonies/Patches or Liquid Culture)|
|Size Range||= 17 nt|
|Yield||= 25 µg of total RNA|
YeaStar RNA Kit