YeaStar RNA Kit

RNA from yeast species using Zymolyase

Highlights

  • Simple: Fast spin-column procedure yields pure yeast RNA without using glass beads or phenol.
  • Versatile: Efficient RNA isolation from a broad spectrum of fungal species susceptible to Zymolyase.
  • High-Quality: Isolated RNA is suitable for use in RT-PCR, Northern blotting, etc.


Description

The YeaStar RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans/, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 µg of total RNA from 1-1.5 ml of cultured cells using innovative Zymo-Spin column technology.

Technical Specifications

Applicable For Isolated RNA is well suited for downstream applications such as Northern blotting, RT-PCR, Next-Generation Sequencing, Microarray, hybridization and other sensitive applications
Elution Volume = 60 &microl DNase/RNase-Free Water
Equipment Microcentrifuge & Heat Block/Bath
Processing Time 30 minutes
Processing Volume = 1.5 ml of Culture (1-5 x 107 cells)
Purity Typical A260/A280 & A260/A230 = 1.8
Sample Source Cell Culture (Colonies/Patches or Liquid Culture)
Size Range = 17 nt
Type Total RNA
Yield = 25 µg of total RNA

Citations

YeaStar RNA Kit

Cat # Name Size
R1002 YeaStar RNA Kit 40 Preps

Documents

Protocol: 
SDS (MSDS):