Urine Conditioning Buffer (UCB) is a urine DNA and RNA stabilizer enabling ambient temperature storage and transport. UCB can be added to any urine collection device and there is no need for refrigeration or specialized equipment. In addition to being a urine DNA and RNA stabilizer, UCB preserves the urine microbiome profile at the time of collection by inhibiting microbial growth and preserving nucleic acid integrity. Nucleic acid from urine samples stored in this DNA and RNA transport and storage medium can be can be isolated directly without reagent removal (compatible with most DNA and RNA purification kits).
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Q1: How long can urine samples be stored after addition of UCB?
Urine samples will be stabilized for a minimum of 30 days at ambient temperature.
Q2: Does UCB preserve nucleic acids like DNA/RNA Shield?
Yes, UCB will preserve DNA and RNA integrity.
Q3: How do I purify DNA/RNA from urine samples stabilized with UCB?
Mix urine thoroughly and centrifuge at 3000 x g for 15 minutes to form a urine pellet. Purify urine nucleic acid directly from urine pellet using Quick-DNA Urine kit(D3061) or Quick-RNA Miniprep Plus kit (R1057, R1058).
