Fast and sensitive ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using SolisGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. It has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low ROX level for signal normalization. SolisGreen® qPCR mixes are suitable for commercial and diagnostic applications and require no additional licensing.

✓ qPCR results 2x faster
✓ fast hot-start (30 sec-3 min)
✓ fast amplification (15-30 sec/kb)
✓ bright and sensitive SolisGreen® dye
✓ suitable for commercial applications
✓ compatible with most cyclers, except high ROX
✓ different product versions: no ROX and ROX
✓ reaction set-up and shipment without ice

SolisFAST® SolisGreen® qPCR Mix is a 5x-concentrated ready-to-use solution optimized for fast, highly sensitive and reproducible dye-based qPCR assays.
Combining novel in silico-designed SolisFAST® DNA Polymerase with fast hot-start, optimized buffer and the SolisGreen® dye, this qPCR Mix offers robust qPCR, accurate target detection, ice free shipping and reaction set-up.

• SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
• qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction
• Reference dye: ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR
•
• Fluorescent dye: SolisGreen® is a nucleic acid dye detected in the FAM or SYBR® Green I channel

Storage:
Shipping:

•

Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.