Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple™ for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.

✓ qPCR results 2x faster
✓ fast hot-start (30 sec-3 min)
✓ fast amplification (15-30 sec/kb)
✓ analyze 1-5 targets in 1 reaction
✓ mix with dUTP and UNG available to prevent carry-over contamination
✓ compatible with most cyclers
✓ different product versions: no ROX, ROX, Purple
✓ reaction set-up and shipment without ice

SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously.
Combining novel in silico-designed SolisFAST® DNA Polymerase with fast hot-start and optimized buffer, this qPCR Mix offers accurate target detection and quantification.

For extra convenience the mix includes dUTP and UNG enzyme that enable UNG treatment to prevent carryover comtamination.

This master mix is developed for TaqMan® probes but is also suitable for other hydrolysis probe types. 

• SolisFAST® DNA Polymerase: In silico- designed analog of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start, and faster extension rates compared to the wild-type Taq DNA polymerase.
• qPCR buffer: Includes 16.5 mM MgCl2 - 1x PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG and additives that maximize efficiency of PCR 
• Reference dye: Purple dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR

Storage: Routine storage at -20ºC until expiry date The mix can be stored at +4ºC for up to 6 months Stability at room temperature (15 − 25ºC) for 1 month Freeze-thaw stability: 30 cycles
Shipping: At room temperature

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Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.