Fast and sensitive ready-to-use 5X-concentrated solution for probe-based qPCR detection of DNA targets using TaqMan® and other hydrolysis probe types. The mix contains a novel SolisFAST® DNA Polymerase with fast hot-start and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases, and is optimised for sensitive detection of up to 5 targets in one reaction. It has three versions to match different instruments and assay requirements. ROX-mix is compatible with qPCR instruments that require low and high ROX level for signal normalization, Purple-mix is compatible with instruments that use Mustang Purple™ for signal normalisation. dUTP and UNG containing mixes are available upon request to prevent carry-over contamination.

✓ qPCR results 2x faster
✓ fast hot-start (30 sec-3 min)
✓ fast amplification (15-30 sec/kb)
✓ analyze 1-5 targets in 1 reaction
✓ mix with dUTP and UNG available to prevent carry-over contamination
✓ compatible with most cyclers
✓ different product versions: no ROX, ROX, Purple
✓ reaction set-up and shipment without ice

SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously.
Combining our novel in silico- designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust qPCR, and accurate target detection combined with ice free shipping and reaction set-up.

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Storage: Routine storage at -20ºC until expiry date The mix can be stored at +4ºC for up to 6 months Stability at room temperature (15 − 25ºC) for 1 month Freeze-thaw stability: 30 cycles
Shipping: At room temperature

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Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.