SolisFAST® Master Mix with UNG

SolisFAST® Master Mix with UNG


SolisFAST® Master Mix with UNG SolisFAST® Master Mix with UNG  Endpoint PCR




  • Fast single- and multiplex endpoint PCR assays!

  • Ultra-fast endpoint PCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target detection. Suitable for UNG treatment to prevent cross-contaminaton

  • ✓ PCR Results in 20 minutes
    ✓ analyze one or multiple targets per reaction
    ✓ contains dUTP and UNG to prevent cross-contamination
    ✓ reliable results in Sanger sequencing applications


Catalog# 24-21-00001-5
Size:5ml (5-pack: 5 x 1 ml/1250 rxn) 1250 rxn 


 Size               Price
 5ml (5-pack: 5 x 1 ml/1250 rxn) Get Quote

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OVERVIEW





A ready-to-use 5X-concentrated solution for fast and ultra-fast singleplex and multiplex endpoint PCR assays. It contains a novel SolisFAST® DNA Polymerase with fast hot-start (30 sec to 3 min) and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. The mix allows amplification of up to 5 kb DNA templates and has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.


✓ PCR results in 20 minutes
✓ fast hot-start (30 sec-3 min)
✓ fast amplification (15-30 sec/kb)
✓ sensitive detection of up to 18 targets per reaction
✓ suitable for templates up to 5 kb
✓ reliable results in Sanger sequencing applications
✓ Ready-to-Load mix available for direct loading to gel
✓ UNG-mix available to prevent carry-over contamination
✓ reaction set-up and shipment without ice


DESCRIPTION

SolisFAST® Master Mix is a 5x-concentrated, ready-to-use solution for fast singleplex and multiplex endpoint PCR assays. The mix allows amplification of up to 5 kb fragments from low complexity DNA templates (e.g. lambda, plasmid DNA), and up to 3 kb from complex DNA templates (e.g. human genomic DNA).
For extra convenience, the mix includes dUTP and UNG enzyme that enable UNG treatment to prevent carryover comtamination.
Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust PCR, accurate target detection, ice-free shipping and reaction set-up. 


KITS COMPONENT

• SolisFAST® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase
• PCR Buffer: Includes 17.5 mM MgCl2 (1x PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR


STORAGE & SHIPPING

Storage: Routine storage at -20ºC until expiry date The mix can be stored at +4ºC for up to 6 months Stability at room temperature (15 − 25ºC) for 1 month Freeze-thaw stability: 30 cycles
Shipping: At room temperature


REAGENT PROVIDED


Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.












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