A ready-to-use 5X-concentrated solution for fast and ultra-fast singleplex and multiplex endpoint PCR assays. It contains a novel SolisFAST® DNA Polymerase with fast hot-start (30 sec to 3 min) and 2-4 times faster extension rates (15-30 sec/kb) compared to the wild-type Taq DNA polymerase (60 sec/kb) as well as HOT FIREPol® and FIREPol® DNA polymerases. The mix allows amplification of up to 5 kb DNA templates and has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.

✓ PCR results in 20 minutes
✓ fast hot-start (30 sec-3 min)
✓ fast amplification (15-30 sec/kb)
✓ sensitive detection of up to 18 targets per reaction
✓ suitable for templates up to 5 kb
✓ reliable results in Sanger sequencing applications
✓ Ready-to-Load mix available for direct loading to gel
✓ UNG-mix available to prevent carry-over contamination
✓ reaction set-up and shipment without ice

SolisFAST® Master Mix is a 5x-concentrated, ready-to-use solution for fast singleplex and multiplex endpoint PCR assays. The mix allows amplification of up to 5 kb fragments from low complexity DNA templates (e.g. lambda, plasmid DNA), and up to 3 kb from complex DNA templates (e.g. human genomic DNA).
Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust PCR, accurate target detection, ice-free shipping and reaction set-up.

The mix can be loaded directly to gel for post PCR analysis.

• SolisFAST® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
• PCR buffer: Includes 17.5 mM MgCl2 (1x PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP),  additives that maximize efficiency of PCR
• Green loading dye: Mix of blue and yellow dye to allow tracking of DNA during electrophoresis.
• The mix is not recommended for use in applications where spectrophotometric measurements are necessary (e.g. Sanger sequencing). Migration speed: blue dye – TBE: ~3.5 kb, TAE: ~4.5 kb; yellow dye – TBE: ~35 bp, TAE: ~45 bp).

Storage: Routine storage at -20ºC until expiry date The mix can be stored at +4ºC for up to 6 months Stability at room temperature (15 − 25ºC) for 1 month Freeze-thaw stability: 30 cycles
Shipping: At room temperature

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Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.