A convenient and sensitive kit for analysis of RNA targets and performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit is optimised for detection of SARS-CoV-2 RNA and it enables the users to develop their own assays based on US CDC and WHO-listed primer and probe sequences, as well as develop custom multi-target tests. The kit contains RiboGrip™ RNase inhibitor and is compatible with most cyclers if signal normalisation is not desired. A kit containg ROX passive reference is available upon request.

✓ optimized for SARS-CoV-2 detection
✓ analyze 1-4 targets in 1 reaction
✓ RNase inhibitor included in kit
✓ wide instrument compatibility
✓ reaction set-up and shipment without ice

SOLIScript® 1-step CoV Kit is optimized for highly sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA by one-step real-time RT-PCR (RT-qPCR).

The Kit is suitable for the detection of up to four targets simultaneously and can be used with all common qPCR cyclers. Follow recommendations specific for a selected SARS-CoV-2 diagnostic panel, and instrument-specific guidelines. Contact us if a passive reference dye is required for signal normalization.

SARS-CoV-2 primers and probes, applicable positive and internal extraction controls are not provided with the Kit and should be supplied by the user.

SOLIScript® 1-step CoV Kit is tested and validated for SARS-CoV-2 detection by diagnostic laboratories. Please see our recommendations for US-CDC and Berlin-Charité protºCols. 

Note! Amplification of more than one target in a single reaction may require considerable optimization, individual assays must be validated prior to multiplexing. For multiplex reactions, design primer pairs with similar annealing temperatures for all targets to be amplified. Self-complementarity of primers should be avoided.

• 5x One-step Probe CoV Mix  with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2)
• 40x One-step SOLIScript® CoV Mix with SOLIScript® Reverse Transcriptase and RiboGrip™ RNase Inhibitor 
• Water, nuclease free

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature

• 40x One-step SOLIScript® CoV Mix 1x50 rxn | 25 µl
• 5x One-step Probe CoV Mix 1x50 rxn | 200 µl
• Water, nuclease free 1x1.25 ml

Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.