A convenient and sensitive kit for analysis of RNA targets and performing cDNA synthesis and a dye-based qPCR in a single tube using SolisGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The Kit includes 3 components: a 40x One-step SOLIScript® Mix for cDNA synthesis, a 5x One-step SolisGreen® Mix for dye-based qPCR, and nuclease-free water. The 5x One-step SolisGreen® Mix contains an internal reference based on ROX dye and is compatible with most qPCR instruments, except those that require high ROX level for signal normalization.

✓ cDNA cynthesis and dye-based qPCR in one tube
✓ high specificity, sensitivity and yield
✓ consistent results with low RNA input
✓ RNase inhibitor included in kit
✓ reaction set-up and shipment without ice

SOLIScript® 1-step SolisGreen® Kit is optimized for highly sensitive and accurate quantification of RNA targets by real-time one-step RT-PCR (RT-qPCR) using dsDNA-intercalating dye detection via the SYBR®/FAM fluorescence channel.
Compatible with ROX-independent and ROX-dependent qPCR cyclers (excluding high-ROX platforms).
The Kit comes in 3 tubes (including water) and contains all the components necessary (except RNA template and primers) to perform cDNA synthesis and qPCR in a single tube.

• 40x One-step SOLIScript® Mix: SOLIScript® Reverse Transcriptase, RiboGrip™ RNase Inhibitor
•
• 5x One-step SolisGreen® Mix: HOT FIREPol® DNA polymerase, dNTPs (dATP, dCTP, dGTP, dTTP), 12.5 mM MgCl2 (1x RT-qPCR solution – 2.5 mM MgCl2), SolisGreen® dsDNA-intercalating dye, passive reference dye based on ROX dye

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature

• 40x One-step SOLIScript® Mix 1x250 rxn | 125 µl
• 5x One-step SolisGreen® Mix 1x250 rxn | 1 ml
• Water, nuclease free 1x5 ml

Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.