SOLIScript® 1-step Multiplex Probe Kit (Purple)
- Sensitive and highly specific cDNA synthesis and qPCR in a single tube
Sensitive and highly specific cDNA synthesis and probe-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
✓ cDNA synthesis up to 60°C for superior specificity
✓ analyze 1-4 targets in 1 reaction
✓ reaction set-up and shipment without dry ice
✓ robust amplification of GC-rich targets
✓ contains dUTP to prevent cross-contamination when used in combination with UNG
✓ RNase inhibitor included in kit
✓ Includes purple reference dye
OVERVIEW
A convenient kit for analysis of RNA targets and performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit is optimised for sensitive quantification of up to 4 targets in one reaction using TaqMan® or other hydrolysis probe types with enhanced amplification of GC-rich targets. It contains RiboGrip™ RNase inhibitor and dUTPs to prevent cross-contamination when used with UNG treatment. It has three versions to match different instruments and assay requirements. ROX-kit is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-kit is compatible with instruments that use Mustang Purple™ for signal normalisation. |
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DESCRIPTION |
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SOLIScript® 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube. |
KITS COMPONENT |
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• 5x One-step Multiplex Probe Mix (Purple) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), Purple dye (If Cy5 dye is used as one of the fluorophores, internal passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available) |
STORAGE & SHIPPING |
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Storage: Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product |
REAGENT PROVIDED |
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• 40x One-step SOLIScript® Mix 1x250 rxn | 125 µl |
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CITATION
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