A convenient kit for analysis of RNA targets and performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit is optimised for sensitive detection of up to 2 targets in one reaction using TaqMan® or other hydrolysis probe types. It contains RiboGrip™ RNase inhibitor and an internal reference based on ROX dye and is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization. The kit is not compatible with Probes detected in ROX/JUN/Texas Red channel.

✓ cDNA synthesis up to 60°C for superior specificity
✓ suitable for assays with 1-2 targets
✓ high specificity and sensitivity
✓ RNase inhibitor included in kit
✓ one kit for all cyclers
✓ reaction set-up and shipment without ice

SOLIScript® 1-step Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays.
It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube

• 5x One-step Multiplex Probe Mix (ROX) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2), internal reference based on ROX dye
• 40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase and RiboGrip™ RNase Inhibitor
• Water, nuclease free

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature

• 40x One-step SOLIScript® Mix 1x50 rxn | 25 µl
• 5x One-step Probe Mix 1x50 rxn | 200 µl
• Water, nuclease free 1x1.25 ml

Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.