A convenient kit for analysis of RNA targets and performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit is optimised for sensitive quantification of up to 4 targets in one reaction using TaqMan® or other hydrolysis probe types with enhanced amplification of GC-rich targets. It contains RiboGrip™ RNase inhibitor and dUTPs to prevent cross-contamination when used with UNG treatment. It has three versions to match different instruments and assay requirements. ROX-kit is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization, Purple-kit is compatible with instruments that use Mustang Purple™ for signal normalisation.

✓ cDNA synthesis up to 60°C for superior specificity
✓ analyze 1-4 targets in 1 reaction
✓ robust amplification of GC-rich targets
✓ contains dUTP to prevent cross-contamination when used in combination with UNG
✓ RNase inhibitor included in kit
✓ wide instrument compatibility
✓ reaction set-up and shipment without ice

SOLIScript® 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube.
Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don't lose in sensitivity compared to singleplex or duplex assays.

• 5x One-step Multiplex Probe Mix with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2)
• 40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase, RiboGrip™ RNase Inhibitor
• Water, nuclease free

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature

• 40x One-step SOLIScript® Mix 1x250 rxn | 125 µl
• 5x One-step Multiplex Probe Mix 1x250 rxn | 1 ml
• Water, nuclease free 1x5 ml

Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.