A highly sensitive ready-to-use 5X reaction mix for dye-based qPCR detection of DNA targets using SolisGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has excellent performance with low template amounts. It contains contains an internal reference based on ROX dye and is compatible with qPCR instruments that require low ROX level for signal normalization. SolisGreen® qPCR mixes are suitable for commercial and diagnostic applications and require no additional licensing.

✓ enhanced performance with low DNA concentrations
✓ bright and sensitive SolisGreen® dye
✓ suitable for commercial applications
✓ one qPCR mix for all cyclers, except high ROX
✓ reaction set-up and shipment without ice

Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate DNA amplification prºCess during PCR. 
HOT FIREPol® SolisGreen® qPCR Mix is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating SolisGreen® dye.
It comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR. The user simply needs to add water, template and primers.
HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

• HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
• 5x qPCR buffer with 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
• dNTPs: dATP, dCTP, dGTP and dTTP
• SolisGreen® dye
• Bovine serum albumin (BSA) to enhance PCR reaction
• Internal reference based on ROX dye

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature

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Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.