A universal ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix is optimised for highly specific results and reduced primer-dimer formation with excellent amplification of GC-rich regions. The Mix contains dUTPs to prevent cross-contamination when used with UNG treatment, and a visible dye to ease reaction set-up. It contains an internal reference based on ROX dye and is compatible with most qPCR instruments, including those that require low and high ROX level for signal normalization.

✓ high sensitivity with low DNA concentrations
✓ robust amplification of GC-rich targets
✓ blue visualization dye to ease pipetting
✓ reduced primer dimer formation
✓ contains dUTP to prevent cross-contamination when used in combination with UNG
✓ one qPCR mix for all cyclers (except capillary)
✓ reaction set-up and shipment without ice

HOT FIREPol® EvaGreen® qPCR Supermix is an optimized 5X reaction mix for dye-based qPCR detection and quantitation of target DNA sequences using EvaGreen® dye and SYBR®/FAM channel of most real-time qPCR instruments.
The Mix contains HOT FIREPol® DNA Polymerase and an passive internal reference dye that is compatible with most qPCR cyclers including high ROX or low ROX reference signal requiering platforms. 
The Mix contains dUTPs to prevent cross-contamination with UNG treatment and visible dye to ease reaction set-up. This dye does not interfere with real-time detection.

• HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
• 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
• dNTPs, including dUTP: mix allows UNG treatment to prevent carryover contamination from previous runs.
• IMPORTANT: UNG is not included in the 5x HOT FIREPol® EvaGreen® Supermix
• EvaGreen® dye
• Internal reference based on ROX dye
• GC-enhancer
• Blue visualisation dye

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature

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Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.