A ready-to-use 5X-concentrated solution for High Resolution Melt (HRM) analysis of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection. The mix has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization.

✓ excellent resolution in HRM assays allows detection of DNA sequence variations
✓ contains sensitive EvaGreen® dye
✓ compatible with most cyclers
✓ different product versions: no ROX, ROX
✓ reaction set-up and shipment without ice

High Resolution Melt (HRM) analysis is fast and cost-effective method for post-PCR analysis to detect single nucleotide polymorphisms (SNP genotyping), genetic mutations and DNA sequence variations.
HOT FIREPol® EvaGreen® HRM Mix is an optimised ready-to-use solution for HRM analysis, incorporating EvaGreen® dye.
It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye according to system requirements. The user simply needs to add water, template and primers.
HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers.

• HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
• 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
• dNTPs: dATP, dCTP, dGTP and dTTP
• EvaGreen® dye
• ROX reference dye
• Bovine serum albumin (BSA) to enhance qPCR reaction

Storage:
Shipping: At room temperature

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Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.