HOT FIREPol® EvaGreen® HRM Mix (no ROX)

HOT FIREPol® EvaGreen® HRM Mix (no ROX)


HOT FIREPol® EvaGreen® HRM Mix (no ROX) HOT FIREPol® EvaGreen® HRM Mix (no ROX)  Dye-based qPCR




  • Optimized ready-to-use solution for High Resolution Melt (HRM) Analysis

  • Optimized ready-to-use solution for High-Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. 

  • ✓ highly sensitive mix for accurate SNP detection
    ✓ sensitive EvaGreen® dye for reliable HRM performance
    ✓ reaction set-up without  ice


Catalog# 08-31-00020
Size:20ml (5000 rxn) 5000 rxn 


 Size               Price
 20ml (5000 rxn) Get Quote

Contact Us




OVERVIEW





A ready-to-use 5X-concentrated solution for High Resolution Melt (HRM) analysis of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection. The mix has two versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization.


✓ excellent resolution in HRM assays allows detection of DNA sequence variations
✓ contains sensitive EvaGreen® dye
✓ compatible with most cyclers
✓ different product versions: no ROX, ROX
✓ reaction set-up and shipment without ice


DESCRIPTION

High Resolution Melt (HRM) analysis is fast and cost-effective method for post-PCR analysis to detect single nucleotide polymorphisms (SNP genotyping), genetic mutations and DNA sequence variations.
HOT FIREPol® EvaGreen® HRM Mix is an optimised ready-to-use solution for HRM analysis, incorporating EvaGreen® dye.
It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers.
HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers.


KITS COMPONENT

• HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
• 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2, 1x PCR solution – 2.5 mM MgCl2
• dNTPs: dATP, dCTP, dGTP and dTTP
• EvaGreen® dye
• Bovine serum albumin (BSA) to enhance qPCR reaction


STORAGE & SHIPPING

Storage:
Shipping: At room temperature


REAGENT PROVIDED


Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.












CITATION






Have you cited this product in a publication?
Let us know so we can reference it here.

Contact Us










We're here to help


Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.


Contact Tech Support







High Quality Guaranteed Product


Our products such as Elisa, Antibodies, Proteins, Peptides are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.


Learn More