A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems.

✓ cost-effective solution for less demanding assays
✓ suitable for most dye-based applications
✓ high sensitivity and specificity
✓ excellent efficiency
✓ compatible with most cyclers
✓ different product versions: no ROX, ROX, capillary
✓ reaction set-up and shipment without ice

Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate DNA amplification prºCess during PCR. In this mix EvaGreen® double-stranded DNA binding dye that is used instead of more widely used SYBR Green I that has similar fluorescence spectra. Compared to SYBR Green I dye EvaGreen® dye shows higher fluorescence level, high sensitivity for detecting low template concentrations and high stability at room temperature. 
HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX) is an optimized ready-to-use solution for dye-based real-time quantitative PCR assays on cyclers that do not require passive reference dye.

• HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
• 5x EvaGreen® qPCR buffer with 12. 5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
• dNTPs: dATP, dCTP, dGTP and dTTP
• EvaGreen® dye

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® qPCR Mix Plus.

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Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.