HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX)
- Cost-effective dye-based qPCR Mix with passive reference dye with ROX
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX and cappillay cyclers.
✓ high sensitivity and specificity
✓ excellent efficiency
✓ reaction set-up and shipment without dry ice
✓ cost-effective solution for wide range of applications
OVERVIEW
A cost-effective ready-to-use 5X-concentrated solution for dye-based qPCR detection of DNA targets using EvaGreen® dsDNA intercalating dye and SYBR®/FAM detection channel. The mix has three versions to match different instruments. The ROX-mix is compatible with qPCR instruments that require low or high ROX level for signal normalization. The capillary-mix is optimised for capillary based systems. |
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DESCRIPTION |
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Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate DNA amplification prºCess during PCR. In this mix EvaGreen® double-stranded DNA binding dye is used instead of more widely used SYBR Green I that has similar fluorescence spectra. Compared to SYBR Green I dye EvaGreen® dye shows higher fluorescence level, high sensitivity for detecting low template concentrations and high stability at room temperature. |
KITS COMPONENT |
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• HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start |
STORAGE & SHIPPING |
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Storage: Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product |
REAGENT PROVIDED |
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CITATION
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