SOLIScript® RT cDNA synthesis KIT

SOLIScript® RT cDNA synthesis KIT


SOLIScript® RT cDNA synthesis KIT SOLIScript® RT cDNA synthesis KIT  cDNA synthesis




  • Superior specificity with gene-specific primers in flexible kit format

  • A flexible and highly specific cDNA synthesis kit contains all the reagents you need for first-strand cDNA synthesis. Individual components enable thorough assay optimization. Note! The kit includes oligo dT primers, SOLIScript® is not recommended with random primers.

  • ✓ superior specificity with gene-specific primers
    ✓ wide reaction temperature 37°C - 60°C
    ✓ reaction set-up and shipment without dry ice
    ✓ in flexible 6-tube kit format


Catalog# 06-35-0000S
Size:50rxn  Free sample


 Size               Price
 50rxn Get Quote

Contact Us




OVERVIEW





Novel in silico-engineered, thermostable reverse transcriptase with reduced RNase H activity. SOLIScript® RT is extremely stable and will remain fully active for up to one month at room temperature.


DESCRIPTION

SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase.
SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C).
SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity.


KITS COMPONENT

• SOLIScript® Reverse Transcriptase
• RiboGrip RNase Inhibitor (40 U/μl) 10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT
• Oligo (dT) Primer (100 μM)
• dNTP MIX (20 mM of each)
• Water, nuclease free


STORAGE & SHIPPING

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature


REAGENT PROVIDED

• SOLIScript® Reverse Transcriptase 1x50 rxn | 50 µl
• RiboGrip RNase Inhibitor 1x50 rxn | 1000 U
• 10x RT Reaction Buffer with DTT 1x125 µl
• dNTP MIX (20 mM of each) 1x2 µmol | 25 µl
• Oligo (dT) Primer 1x50 µl
• Water, nuclease free 1x1.25 ml


Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.












CITATION






Have you cited this product in a publication?
Let us know so we can reference it here.

Contact Us










We're here to help


Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.


Contact Tech Support







High Quality Guaranteed Product


Our products such as Elisa, Antibodies, Proteins, Peptides are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.


Learn More