SOLIScript® KIT

SOLIScript® KIT


SOLIScript® KIT SOLIScript® KIT  cDNA synthesis




  • Reverse transcriptase with high thermostability and superior specificity

  • A flexible and highly specific cDNA synthesis kit contains all the reagents you need for first-strand cDNA synthesis. Individual components enable thorough assay optimization. Note! The kit includes oligo(dT) primers, SOLIScript® is not recommended with random primers.

  • ✓ superior performance with gene-specific primers
    ✓ reaction temperatures from 37°C to 60°C
    ✓ reaction set-up and shipment at room temperature
    ✓ NB! not compatible with random primers


Catalog# 06-33-00050
Size:50rxn  


 Size               Price
 50rxn Get Quote

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OVERVIEW





Novel in silico-engineered, thermostable reverse transcriptase with reduced RNase H activity. SOLIScript® RT is extremely stable and will remain fully active for up to one month at room temperature.


DESCRIPTION

SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase.
SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatures (up to 60°C).
SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains an RNase H domain with reduced activity.


KITS COMPONENT

• SOLIScript® Reverse Transcriptase
• 10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT


STORAGE & SHIPPING

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature


REAGENT PROVIDED

• SOLIScript® Reverse Transcriptase 1x50 rxn | 50 µl
• 10x RT Reaction Buffer with DTT 1x125 µl


Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.












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