25 mM MgCl2
- Stock solution for PCR assay optimization
Additive for adjusting Mg2+ concentration in PCR assay.
✓ additive for (q)PCR
✓ additive for RT-(q)PCR
OVERVIEW
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Magnesium Chloride (MgCl₂) is an important component of PCR reactions. Concentration of MgCl₂ should be optimized according to reaction conditions (primer, template, dNTP, polymerase concentration). |
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DESCRIPTION |
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MgCl2 solution is used for adjusting Mg2+ concentration in PCR assay. It can be used in combination with PCR buffers that come with FIREPol® and HOT FIREPol® which are all MgCl2 free). In most applications, a final concentration of 1,5 mM MgCl2 is optimal for a satisfactory yield. In some cases, MgCl2 titration is needed for a better yield and specificity. |
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KITS COMPONENT |
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STORAGE & SHIPPING |
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Storage: Routine storage: -20ºC Temporary storage at room temperature has no detrimental effects on the quality of this reagent. |
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REAGENT PROVIDED |
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CITATION
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