HOT FIREPol® MultiPlex Mix with 10 mM MgCl2

HOT FIREPol® MultiPlex Mix with 10 mM MgCl2

HOT FIREPol® MultiPlex Mix with 10 mM MgCl2 HOT FIREPol® MultiPlex Mix with 10 mM MgCl2  Endpoint PCR

  • Hot-start master mix for high throughput applications

  • Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays

  • ✓ analyze multiple targets per reaction
    ✓ increased sensitivity and yield
    ✓ prevents primer dimer formation
    ✓ reaction set-up and shipment without dry ice

Catalog# 04-34-00S20
Size:0.1ml (25 rxn) 25 rxn Free sample

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A ready-to-use 5X-concentrated solution for singleplex and multiplex endpoint PCR assays. It contains a hot-start Taq DNA polymerase HOT FIREPol® and allows amplification of up to 5 kb DNA templates. The mix has two versions - regular and Ready-to-Load mix which includes loading dyes for direct loading to gel.

✓ sensitive detection of up to 18 targets per reaction
✓ suitable for templates up to 5 kb
✓ increased sensitivity and yield
✓ reduced primer dimer formation
✓ Ready-to-Load mix available
✓ reaction set-up and shipment without ice


HOT FIREPol® MultiPlex Mix is a premixed ready-to-use solution containing all reagents required for hot-start multiplex PCR (except template, primers and water) and an additional compound needed for direct loading onto agarose gel.
Generated PCR products are compatible with TA cloning prºCedures.


• HOT FIREPol® DNA polymerase
• 5x Multiplex Buffer with 10 mM MgCl2 (1x PCR solution – 2 mM MgCl2)
• dNTPs 
• Compound that increases sample density for direct loading (loading dyes not included)


Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature


Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.


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