A ready-to-use 5X-concentrated solution for endpoint PCR assays, optimised for GC-rich templates. It contains a hot-start Taq DNA polymerase HOT FIREPol® and allows amplification of up to 5 kb DNA templates. Separate tubes of 25 mM MgCl₂ and an additive for difficult templates (100% DMSO) are included with the mix.

✓ excellent amplification with templates up to 79% GC content
✓ suitable for templates up to 5 kb
✓ vials of 100% DMSO and 25 mM MgCl₂ enable flexibility in reaction optimization
✓ reaction set-up and shipment without ice

HOT FIREPol® GC Master Mix x that has been developed for working with difficult GC-rich templates and DNA secondary structures. It shows excellent amplification with templates up to 79% GC content
The master mix contains hot-start Taq polymerase HOT FIREPol®, MgCl2, dNTPs and a special buffer for high yield GC-rich amplification.
Extra vials of 100% DMSO and 25 mM MgCl2 enable flexibility in reaction optimization. 

• HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
• 5x HOT FIREPol® GC Buffer with 7.5 mM MgCl2: 1x PCR solution –1.5 mM MgCl2
• dNTPs: dATP, dCTP, dGTP and dTTP
• Bovine serum albumin (BSA) to enhance PCR reaction
• In separate vials:
• 100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures.
• 25 mM MgCl2 for further optimization

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature

• HOT FIREPol® GC Master Mix 1x0.1 ml | 25 rxn
• 25 mM MgCl2 1x0.1 ml
• DMSO 100% 1x0.1 ml

Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.