A ready-to-use 5X-concentrated solution for more demanding endpoint PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced fidelity and performance. The mix has regular and Ready-to-Load version and different MgCl₂ options for easier optimization.

✓ increased yield, sensitivity and specificity
✓ up to 5x higher fidelity
✓ suitable for templates up to 5 kb
✓ reduced primer dimer formation
✓ Ready-to-Load mix available
✓ reaction set-up and shipment without ice

HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water).
HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to regular Taq polymerase.
In addition, the mix also allows amplification of longer fragments.
Generated PCR products are compatible with blunt-end and T/A cloning prºCedures.

• HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
• Proofreading enzyme: to enhance fidelity
• 5x Blend Master Mix Buffer with 12. 5 mM MgCl2 1x PCR solution – 1.5 mM MgCl2
• 1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
• Bovine serum albumin (BSA) to enhance PCR reaction

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of HOT FIREPol® Blend Master Mix
Shipping: At room temperature

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Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.