HOT FIREPol® Blend Master Mix Ready to Load with 7.5 mM MgCl2

HOT FIREPol® Blend Master Mix Ready to Load with 7.5 mM MgCl2


HOT FIREPol® Blend Master Mix Ready to Load with 7.5 mM MgCl2 HOT FIREPol® Blend Master Mix Ready to Load with 7.5 mM MgCl2  Endpoint PCR




  • Ready to load hot-start PCR master mix for more demanding PCR assays

  • Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions. Includes two tracking dyes.

  • ✓ increased yield, sensitivity and specificity
    ✓ up to 5x higher fidelity
    ✓ suitable for templates up to 5 kb
    ✓ reduced primer dimer formation
    ✓ load directly on gel after PCR


Catalog# 04-25-00115-5
Size:5ml (5-pack: 5 x 1 ml/1250 rxn) 1250 rxn 


 Size               Price
 5ml (5-pack: 5 x 1 ml/1250 rxn) Get Quote

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OVERVIEW





A ready-to-use 5X-concentrated solution for more demanding endpoint PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced fidelity and performance. The mix has regular and Ready-to-Load version and different MgCl₂ options for easier optimization. Ready-to-Load mix ncludes loading dyes for direct loading to gel.


✓ increased yield, sensitivity and specificity
✓ up to 5x higher fidelity
✓ suitable for templates up to 5 kb
✓ reduced primer dimer formation
✓ Ready-to-Load mix available
✓ reaction set-up and shipment without ice


DESCRIPTION

HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water), additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.
HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to regular Taq polymerase.
In addition, the mix also allows amplification of longer fragments.
Generated PCR products are compatible with blunt-end and T/A cloning prºCedures.
HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to regular Taq polymerase.


KITS COMPONENT

• HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabeling hot-start
• Proofreading enzyme: to enhance fidelity
• 5x Blend Master Mix Buffer with 7. 5 mM MgCl2: 1x PCR solution – 1.5 mM MgCl2
• 1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
• Bovine serum albumine (BSA) to enhance PCR reaction
• Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment
• Yellow dye: migration rate in excess of primers in 1% agarose gel: <35-45 bp
• Compound that increases sample density for direct loading


STORAGE & SHIPPING

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature


REAGENT PROVIDED


Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.












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