A ready-to-use 5X-concentrated solution for routine endpoint PCR assays. It contains a thermostable Taq DNA polymerase FIREPol® and allows amplification of up to 5 kb DNA templates.

✓ all-in-one master mix format reduces pipetting errors and saves time
✓ suitable for templates up to 5 kb
✓ Ready-to-Load format for direct loading to gel
✓ reaction set-up and shipment without ice

Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs and buffer with detergent. You just need to add template, primers and water.
Ready to Load format also includes a compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.
We recommend using FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.

• FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase
• 5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
• 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
• 1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
• Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment
• Yellow dye: migration equivalent to 35-45 bp DNA fragment
• Compound that increases sample density for direct loading

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature

•

Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.