A ready-to-use 5X-concentrated solution for routine endpoint PCR assays. It contains a thermostable Taq DNA polymerase FIREPol® and allows amplification of up to 5 kb DNA templates.

✓ all-in-one master mix format reduces pipetting errors and saves time
✓ suitable for templates up to 5 kb
✓ reaction set-up and shipment without ice

Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs and buffer with detergent. You just need to add template, primers and water.
We recommend using FIREPol® Master Mix without ready to load feature in all PCR applications where spectro-photometric measurements (absorbance or fluorescence) are necessary post-PCR because loading dyes can interfere with these applications.

• FIREPol® DNA polymerase: highly processive thermostable DNA polymerase
• 5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
• 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
• 1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature

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Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.