Chemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only after heat treatment at 95°C which prevents any unspecific polymerase activity at lower temperatures during reaction set-up. HOT FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl₂ and an additive for difficult templates. HOT FIREPol® 10x Buffer B2 contains non-ionic detergent suppressing inhibitory effects of the trace of DNA extraction buffers and enhancing PCR yield and efficiency.

✓ increased specificity and sensitivity
✓ reduced primer dimer formation
✓ suitable for TA cloning
✓ reaction buffer with and without detergent included
✓ Solution S included in a separate vial for GC-rich templates
✓ MgCl₂ included in a separate vial
✓ reaction set-up and shipment without ice

HOT FIREPol® is a chemically modified FIREPol® DNA Polymerase.
HOT FIREPol® is inactive at room temperature and is activated by a 15 min incubation step at 95°C. This enables hot-start PCR and minimizes mispriming and extension from non-specifically annealed primers and primer-dimers. Recommended for routine applications (fragment up to 5 kb).
Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning.
The fidelity of HOT FIREPol® is similar to a regular Taq DNA Polymerase (error rate per nucleotide app. 2.5 x10-5).

• HOT FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers.
• HOT FIREPol® 10x Buffer B1 (without Mg2+ and detergent): 0.7 M Tris-HCl, 0.175 M (NH4)2SO4.
• HOT FIREPol® 10x Buffer B2 (without Mg2+): 0.7 M Tris-HCl, 0.175 M (NH4)2SO4, 0.2% w/v Tween-20. HOT FIREPol® 10x Buffer B2 contains non-ionic detergent suppressing inhibitory effects of the trace of DNA extraction buffers and enhancing PCR yield and efficiency.
• 25 mM MgCl2
• 10x Solution S is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined final concentration (1x, 2x or 3x solution). 10x Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplification ºCcurs.

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature

• HOT FIREPol® DNA Polymerase 1x100 U | 20 µl
• 10x Solution S 1x0.1 ml
• 25 mM MgCl2 1x0.5 ml
• HOT FIREPol® 10x Buffer B1 1x0.5 ml
• HOT FIREPol® 10x Buffer B2 1x0.5 ml

Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.