FIREPol® DNA Polymerase

FIREPol® DNA Polymerase


FIREPol® DNA Polymerase FIREPol® DNA Polymerase  Endpoint PCR




  • Highly processive, thermostable Taq DNA polymerase

  • FIREPol® is a highly processive, thermostable Taq DNA polymerase with unique 30-day stability at room temperature.

  • ✓ robust amplification in routine applications
    ✓ suitable for templates up to 5 kb
    ✓ reaction set-up and shipment without ice
    ✓ TA cloning


Catalog# 01-01-01000
Size:1000U 200 µl 


 Size               Price
 1000U Get Quote

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OVERVIEW





A genetically modified thermostable Taq DNA polymerase that provides robust and reproducible results. FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl2 and an additive for difficult templates. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency.


✓ robust amplification for routine applications
✓ suitable for templates up to 5 kb
✓ suitable for TA cloning
✓ reaction buffers with and without detergent included
✓ Solution S included in a separate vial for GC-rich templates
✓ MgCl2 included in a separate vial
✓ reaction set-up and shipment without ice


DESCRIPTION

FIREPol® is a highly processive, thermostable DNA Polymerase. Due to its genetic modifications, it has enhanced stability at room temperature with no activity loss for up to 1 month.
Recommended for routine applications (fragment up to 3 kb from genomic DNA).
Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning.
The fidelity of FIREPol® is similar to a regular Taq DNA Polymerase (error rate per nucleotide ca 2.5 x10-5).


KITS COMPONENT

• FIREPol® DNA Polymerase: (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers.
• FIREPol® 10x Buffer B (without Mg2+ ): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20. FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency.
• FIREPol® 10x Buffer BD (without Mg2+ and detergent): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4.
• 25 mM MgCl2
• 10x Solution S is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates).


STORAGE & SHIPPING

Storage: Routine storage: -20ºC Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the product
Shipping: At room temperature


REAGENT PROVIDED

• FIREPol® DNA Polymerase 1x1000 U | 200 µl
• 10x Reaction Buffer B 1x5 ml
• 10x Reaction Buffer BD 1x5 ml
• 10x Solution S 1x0.5 ml
• 25 mM MgCl2 1x5 ml


Some applications of this product may require a license which is not provided by the purchase of this product.

**For research use only.












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