Read over 350 inspirational, peer-reviewed publications and discover the power of RPA.
Genome-informed diagnostics for specific and rapid detection of Pectobacterium species using recombinase polymerase amplification coupled with a lateral flow device.
Author: Ahmed FA, Larrea-Sarmiento A, Alvarez AM, Arif M.
Recombinase polymerase amplification (RPA) combined with a lateral flow device (LFD) was developed for specific detection of Pectobacterium sp. directly from infected plant materials with no need for DNA isolation. The specificity of RPA-LFD was tested with 26 Pectobacterium sp. …
Reverse transcription recombinase polymerase amplification with lateral flow dipsticks for detection of influenza A virus and subtyping of H1 and H3.
Author: Sun N, Wang W, Wang J, Yao X, Chen F, Li X, Yinglei Y, Chen B.
Three reverse transcription recombinase polymerase amplification assays with lateral flow dipsticks (RT-RPA-LFD) were developed for identification of the matrix and hemagglutinin (HA) genes to detect influenza A virus and distinguish subtypes H1 and H3. The sensitivity of RT-RPA-LFD assays is …
Rapid detection of bovine viral diarrhea virus using recombinase polymerase amplification combined with lateral flow dipstick assays in bulk milk.
Author: Hou P, Zhao G, Wang H, He C, He H.
The aim of this study was to develop a novel isothermal recombinase polymerase amplification (RPA) method combined with a lateral flow dipstick (LFD), for rapid detection of BVDV. The detection limit of this assay was 20 copies per reaction, and …
Rapid detection of Listonella anguillarum by recombinase polymerase amplification technique.
Author: JianHu P, LongLiang Q, HaiLong H, ChenYang D, Peng Z, JiLin X.
In this study, several pairs of primers and an exo-probe targeting the zinc metalloprotease gene (empA) of Listonella anguillarum was designed and a rapid real-time detection method for Listonella anguillarum based on recombinase polymerase amplification (RPA) was established by optimizing …
Rapid detection of mcr-1 by recombinase polymerase amplification
Author: Xu J, Wang X, Yang L, Kan B, Lu X.
RPA detection based on the mcr-1 gene was successfully applied in our study. The plasmid-mediated mcr-1 gene conferring colistin drug resistance has a strong ability to spread, suggesting the need to further strengthen the detection of this resistance gene in …
Rapid detection of cyanobacteria by recombinase polymerase amplification combined with lateral flow strips.
Author: Li J, Wang C, Yu X, Lin H, Hui C, Shuai L, Zhang S.
Cyanobacteria are one of the major groups of algae causing algal blooms. In this study a rapid method for detecting Cyanobacteria was developed using a recombinase polymerase amplification (RPA) method coupled with lateral flow (LF) strips. The method developed in …
Reverse Transcription–Recombinase Polymerase Amplification Assay for the Detection of Sugarcane Streak Mosaic Virus in Sugarcane.
Author: Feng XY, Shen LB, Wang WZ, Wang JG, Cao ZY, Zhang SZ.
In this study, a novel isothermal assay was established on the basis of reverse transcription–recombinase polymerase amplification (RT-RPA) and evaluated in terms of its ability to detect SCSMV. Overall, the RT-RPA assay was specific, sensitive, reliable, and rapid for SCSMV …
Detection of Fusarium oxysporum f. sp. fragariae from infected strawberry plants.
Author: Burkhardt A, Henry PM, Koike ST,Gordon T, Martin FN.
In this study, next-generation sequencing and comparative genomics were used to identify a unique genetic locus that can detect all the somatic compatibility groups of F. oxysporum f. sp. fragariae identified in California. This locus was used to develop a …
Recombinase polymerase amplification assay for rapid detection of the root-knot nematode Meloidogyne enterolobii.
Author: Subbotin SA.
RPA assays were developed targeting the IGS rRNA gene of the pacara earpod tree root-knot nematode, Meloidogyne enterolobii. The RPA assays using TwistAmp® Basic and TwistAmp® exo kits allowed detection of M. enterolobii from gall tissues and crude nematode extracts …
Development and application of a simple recombinase polymerase amplification assay for rapid point-of-care detection of feline herpesvirus type 1.
Author: Liu MZ, Han XH, Yao LQ, Zhang WK, Liu BS, Chen ZL.
A recombinase polymerase amplification (RPA) assay (RPA-LFD-FHV) combined with a lateral flow dipstrip (LFD) was developed that uses human body heat for incubation. The RPA-FLD-FHV assay is a simple, rapid, and reliable method for point-of-care diagnosis of FHV-1 infection.