Read over 350 inspirational, peer-reviewed publications and discover the power of RPA.

Every year more and more scientists are finding out that RPA really works. Over 350 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren’t possible with PCR.
*Biolinkk takes no responsibility for the content of the publications or their author/s.

Genome-informed diagnostics for specific and rapid detection of Pectobacterium species using recombinase polymerase amplification coupled with a lateral flow device.

Author: Ahmed FA, Larrea-Sarmiento A, Alvarez AM, Arif M.

Recombinase polymerase amplification (RPA) combined with a lateral flow device (LFD) was developed for specific detection of Pectobacterium sp. directly from infected plant materials with no need for DNA isolation. The specificity of RPA-LFD was tested with 26 Pectobacterium sp. …

Reverse transcription recombinase polymerase amplification with lateral flow dipsticks for detection of influenza A virus and subtyping of H1 and H3.

Author: Sun N, Wang W, Wang J, Yao X, Chen F, Li X, Yinglei Y, Chen B.

Three reverse transcription recombinase polymerase amplification assays with lateral flow dipsticks (RT-RPA-LFD) were developed for identification of the matrix and hemagglutinin (HA) genes to detect influenza A virus and distinguish subtypes H1 and H3. The sensitivity of RT-RPA-LFD assays is …

Rapid detection of bovine viral diarrhea virus using recombinase polymerase amplification combined with lateral flow dipstick assays in bulk milk.

Author: Hou P, Zhao G, Wang H, He C, He H.

The aim of this study was to develop a novel isothermal recombinase polymerase amplification (RPA) method combined with a lateral flow dipstick (LFD), for rapid detection of BVDV. The detection limit of this assay was 20 copies per reaction, and …

Rapid detection of Listonella anguillarum by recombinase polymerase amplification technique.

Author: JianHu P, LongLiang Q, HaiLong H, ChenYang D, Peng Z, JiLin X.

In this study, several pairs of primers and an exo-probe targeting the zinc metalloprotease gene (empA) of Listonella anguillarum was designed and a rapid real-time detection method for Listonella anguillarum based on recombinase polymerase amplification (RPA) was established by optimizing …

Rapid detection of mcr-1 by recombinase polymerase amplification

Author: Xu J, Wang X, Yang L, Kan B, Lu X.

RPA detection based on the mcr-1 gene was successfully applied in our study. The plasmid-mediated mcr-1 gene conferring colistin drug resistance has a strong ability to spread, suggesting the need to further strengthen the detection of this resistance gene in …

Rapid detection of cyanobacteria by recombinase polymerase amplification combined with lateral flow strips.

Author: Li J, Wang C, Yu X, Lin H, Hui C, Shuai L, Zhang S.

Cyanobacteria are one of the major groups of algae causing algal blooms. In this study a rapid method for detecting Cyanobacteria was developed using a recombinase polymerase amplification (RPA) method coupled with lateral flow (LF) strips. The method developed in …

Reverse Transcription–Recombinase Polymerase Amplification Assay for the Detection of Sugarcane Streak Mosaic Virus in Sugarcane.

Author: Feng XY, Shen LB, Wang WZ, Wang JG, Cao ZY, Zhang SZ.

In this study, a novel isothermal assay was established on the basis of reverse transcription–recombinase polymerase amplification (RT-RPA) and evaluated in terms of its ability to detect SCSMV. Overall, the RT-RPA assay was specific, sensitive, reliable, and rapid for SCSMV …

Detection of Fusarium oxysporum f. sp. fragariae from infected strawberry plants.

Author: Burkhardt A, Henry PM, Koike ST,Gordon T, Martin FN.

In this study, next-generation sequencing and comparative genomics were used to identify a unique genetic locus that can detect all the somatic compatibility groups of F. oxysporum f. sp. fragariae identified in California. This locus was used to develop a …

Recombinase polymerase amplification assay for rapid detection of the root-knot nematode Meloidogyne enterolobii.

Author: Subbotin SA.

RPA assays were developed targeting the IGS rRNA gene of the pacara earpod tree root-knot nematode, Meloidogyne enterolobii. The RPA assays using TwistAmp® Basic and TwistAmp® exo kits allowed detection of M. enterolobii from gall tissues and crude nematode extracts …

Development and application of a simple recombinase polymerase amplification assay for rapid point-of-care detection of feline herpesvirus type 1.

Author: Liu MZ, Han XH, Yao LQ, Zhang WK, Liu BS, Chen ZL.

A recombinase polymerase amplification (RPA) assay (RPA-LFD-FHV) combined with a lateral flow dipstrip (LFD) was developed that uses human body heat for incubation. The RPA-FLD-FHV assay is a simple, rapid, and reliable method for point-of-care diagnosis of FHV-1 infection.