Read over 350 inspirational, peer-reviewed publications and discover the power of RPA.

Every year more and more scientists are finding out that RPA really works. Over 350 peer-reviewed publications* have been written as people discover the benefits of RPA. See the publications on this page to give you inspirational ideas of how you could use RPA for experiments that just aren’t possible with PCR.
*Biolinkk takes no responsibility for the content of the publications or their author/s.

Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay.

Author: Wu T, Wu H, Zhao K, Hu C, Ge Y, Zhu X, Zhang X, Zhou M, Zhu F, Cui L.

As an important component of the causative agent of respiratory tract infections, enteric and eye infections, Human mastadenoviruses (HAdVs) species B spread easily in the crowd. In this study, an RPA assay was developed for rapidly detecting HAdVs species B …

A real-time recombinase polymerase amplification assay for the rapid detection of Vibrio harveyi.

Author: Pang J, Wang Q, Fei Y, Zhu P, Qiao L, Huang H, Dang C, Gao W.

Vibrio harveyi is a pathogen that infects fish and shellfish worldwide, causing severe economic losses for the aquaculture industry. As the early diagnosis of V. harveyi infection is crucial to disease surveillance and prevention in cultured marine animals, a fast …

Rapid and visual detection of Trichinella spp. using a lateral flow strip-based recombinase polymerase amplification (LF-RPA) assay.

Author: Li TT, Wang JL, Zhang NZ, Li WH, Yan HB, Li L, Jia WZ, Fu BQ.

Accurate diagnosis of Trichinella spp. infection in domestic animals is crucial for the effective prevention and control of human trichinellosis. In the present study, a simple, rapid and accurate diagnostic assay was developed combining recombinase polymerase amplification and a lateral …

Multilocus characterization, gene expression analysis of putative immunodominant protein coding regions, and development of recombinase polymerase amplification assay for detection of ‘Candidatus Phytoplasma pruni’ in Prunus avium.

Author: Villamor DEV, Eastwell K.

Recent escalation of WX disease in Washington State prompted the development of a rapid detection assay based on recombinase-polymerase amplification (RPA) to facilitate timely removal and replacement of diseased trees. This study reports a reliable RPA assay targeting putative immunodominant …

Rapid detection of Cucumber green mottle mosaic virus by reverse transcription recombinase polymerase amplification.

Author: Zeng R, Luo J, Gao S, Xu L, Song Z, Dai F.

Reverse transcription recombinase polymerase amplification (RT-RPA) for detection of Cucumber green mottle mosaic virus (CGMMV) was developed in this study. It was found to be specific, with a limit of detection of 0.5 pg of total CGMMV RNA. CGMMV on inoculated …

Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need in Sri Lanka.

Author: Gunaratna G, Manamperi A, Bohlken-Fascher S, Wickremasinge R, Gunawardena K, Yapa B, Pathirana N, Pathirana H, de Silva N, Sooriyaarachchi M, Deerasinghe T, Mondal D, Ranasinghe S, Abd El Wahed A.

In this study, a mobile suitcase laboratory applying novel extraction (SpeedXtract) and isothermal amplification and detection (recombinase polymerase amplification assay, RPA) methods were evaluated for the diagnosis of cutaneous leishmaniasis in Sri Lanka.

An integrated multi-molecular sensor for simultaneous BRAFV600E protein and DNA single point mutation detection in circulating tumour cells.

Author: Dey S, Koo K, Wang Z, Sina AAI, Wuethrich A, Trau M.

This study presents an integrated multi-molecular sensor (IMMS) for an entire sample-to-answer workflow from melanoma cell capture to simultaneous quantification of both intracellular BRAFV600E DNA and protein levels on a single platform.

Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis.

Author: Zhao G, Hou P, Huan Y, He C, Wang H, He H.

Mycoplasma bovis (M. bovis) is a major etiological agent of bovine mycoplasmosis around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. …

Real-time recombinase polymerase amplification assay for the rapid and sensitive detection of Campylobacter jejuni in food samples.

Author: Geng Y, Liu G, Lui L, Deng Q, Zhao L, Sun XX, Wang J, Zhao B, Wang J.

In this study, a real-time RPA assay was developed so as to achieve the rapid and efficient detection of C. jejuni by targeting the hipO gene. The specificity and sensitivity of real-time RPA was validated and the practical applicability of …

Development and evaluation of a broadly reactive reverse transcription recombinase polymerase amplification assay for rapid detection of murine norovirus.

Author: Ma L, Zeng F, Cong F, Huang B, Zhu Y, Wu M, Xu F, Yuan W, Huang R, Guo P.

Murine norovirus (MNV) is recognized as the most prevalent viral pathogen in captive mouse colonies. The rapid detection assay for MNV would be a useful tool for monitoring and preventing MNV infection. A recombinase polymerase amplification (RPA) assay was established …