- Quick and Reliable: 30 minute recovery of purified RNA fragments from agarose gels.
- Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
- High Recovery: ≥ 80% recovery for RNA > 500 nt.
The Zymoclean Gel RNA Recovery Kit provides a quick and efficient purification method for recovery of RNA fragments from agarose gels. The procedure combines a unique, single-step agarose dissolving/RNA binding buffer with Zymo-Spin column technology to yield high quality, purified RNA in just minutes. The purified RNA is eluted into small volumes of DNase/RNase-free water for highly concentrated samples suitable for subsequent RNA-based manipulations. Compatible with MOPS, TAE, and TBE buffered agarose gels (formaldehyde up to 2.0%).
|Equipment||Microcentrifuge, 37°C-65°C heat source|
|Purity||RNA is ready for all subsequent analysis and molecular manipulation|
|Sample Source||Single- or double-stranded RNA fragments (≥200 nt) resolved in TAE TBE, and MOPS buffered agarose gels. Compatible with formaldehyde to 2.0% (final conc.)|
|Size Range||RNA fragments ≥500 nt (≥80% recovery)|
|Yield||10 µg RNA (binding capacity), ≥6 µl (elution volume)|
Q1: Are the columns and wash buffer interchangeable with the RCC-5 Kit?
Q2: Can the Zymoclean be used for samples in solution (without running on a gel)?
Q3: What is the maximum weight of gel slice I can use?
Q4: I ran out of RNA Wash Buffer. Can I use something else?
Q5: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?
Zymoclean Gel RNA Recovery Kit