Description
The Zymoclean Gel RNA Recovery Kit provides a quick and efficient purification method for recovery of RNA fragments from agarose gels. The procedure combines a unique, single-step agarose dissolving/RNA binding buffer with Zymo-Spin column technology to yield high quality, purified RNA in just minutes. The purified RNA is eluted into small volumes of DNase/RNase-free water for highly concentrated samples suitable for subsequent RNA-based manipulations. Compatible with MOPS, TAE, and TBE buffered agarose gels (formaldehyde up to 2.0%).
Technical Specifications
Equipment |
Microcentrifuge, 37°C-65°C heat source |
Purity |
RNA is ready for all subsequent analysis and molecular manipulation |
Sample Source |
Single- or double-stranded RNA fragments (≥200 nt) resolved in TAE TBE, and MOPS buffered agarose gels. Compatible with formaldehyde to 2.0% (final conc.) |
Size Range |
RNA fragments ≥500 nt (≥80% recovery) |
Yield |
10 µg RNA (binding capacity), ≥6 µl (elution volume) |
Product FAQ
Q1: Are the columns and wash buffer interchangeable with the RCC-5 Kit?
Yes, the Zymo-Spin IC Columns and RNA Wash Buffer are interchangeable.
Q2: Can the Zymoclean be used for samples in solution (without running on a gel)?
No, it’s not optimized for samples in solution. High efficiency recovery is not guaranteed and the success of the purification can be variable.
Q3: What is the maximum weight of gel slice I can use?
Q4: I ran out of RNA Wash Buffer. Can I use something else?
Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
Q5: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?
Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.