The ZR small-RNA PAGE Recovery Kit provides an easy and efficient method for the extraction of high quality small RNAs from polyacrylamide gels (native and/or denatured). The ZR small-RNA™ PAGE Recovery Kit is a refinement of the “crush and soak” method that incorporates a unique buffer system together with Zymo-Spin column technologies for improved recovery and added convenience. The recovered RNA can be concentrated into volumes as small as 6 µl, making it ideal for many downstream enzymatic reactions and manipulations.
Q1: What is the recovery rate?
The recovery rate for fragments 17 to 28 nucleotides is =50 %.
Q2: Can the kit be used to recover DNA fragments run on a PAGE gel?
Yes, the kit is compatible with both single- or double-stranded RNA (and DNA) fragments.
Q3: Can the kit be used to purify DNA/RNA fragments > 200 nucleotides run on a PAGE gel?
Yes. Most customer’s do not use PAGE gels for to visualize fragments > 200 nucleotides, but it will be compatible with the kit.
Q4: Is the kit compatible with TBE-Urea PAGE?
Yes, this kit is compatible with TBE-Urea PAGE gels.
Q5: What is the maximum amount of gel that can be processed per prep?
There is no designated upper limit, however it is always more optimal to keep the amount of excess gel as low as possible to ensure that the gel slice completely dissolves prior to proceeding with the purification procedure.
Q6: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?
Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.
