ZR small-RNA PAGE Recovery Kit

RNA (and DNA) from PAGE gels


  • Efficient: Recovery of ss- or ds- RNA (and DNA) fragments (17-200 nt) from up to 20% (w/v) polyacrylamide gels.
  • Concentrated: Up to 10 µg sample in = 6 µl elution.
  • Compatible with up to 25% (w/v) polyacrylamide.


The ZR small-RNA PAGE Recovery Kit provides an easy and efficient method for the extraction of high quality small RNAs from polyacrylamide gels (native and/or denatured). The ZR small-RNA™ PAGE Recovery Kit is a refinement of the “crush and soak” method that incorporates a unique buffer system together with Zymo-Spin column technologies for improved recovery and added convenience. The recovered RNA can be concentrated into volumes as small as 6 µl, making it ideal for many downstream enzymatic reactions and manipulations.

Technical Specifications

Equipment Microcentrifuge, 37°C-65°C heat source, dry ice or -80°C freezer
Purity RNA is ready for all subsequent analysis and molecular manipulation
Sample Source RNA or DNA fragments (17-200 nt) resolved in PAGE gels (up to 25%), also compatible with TBE-, denatured, urea PAGE gels. Compatible with ethidium bromide or ssRNA-specific dyes (e.g. GelStar).
Size Range RNAs 17-200 nt (fragments 17-28 nt is ≥50 % recovery)
Yield 10 µg RNA (binding capacity), ≥6 µl (elution volume)

Product FAQ

Q1: What is the recovery rate?

Q2: Can the kit be used to recover DNA fragments run on a PAGE gel?

Q3: Can the kit be used to purify DNA/RNA fragments > 200 nucleotides run on a PAGE gel?

Q4: Is the kit compatible with TBE-Urea PAGE?

Q5: What is the maximum amount of gel that can be processed per prep?

Q6: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?


Kit Components

ZR small-RNA PAGE Recovery Kit


Cat # Name Size
R1070 ZR small-RNA PAGE Recovery Kit 20 Preps