Viral DNA and RNA from all biological fluids

Highlights

Viral RNA: Compatible with plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, biopsies, swabs, feces, samples in DNA/RNA Shield, etc.
Streamlined Workflow: Sample inactivation and easy one-step lysis enables fast processing.
High Sensitivity: Optimized for low viral copy detection for Next-Gen
Sequencing and RT-qPCR.

Description

The Quick-RNA Viral Kit is a quick viral RNA purification kit for viral RNA from plasma, serum, cell culture media, cellular suspensions, urine, blood, saliva and any other biological samples stored in DNA/RNA Shield™.
DNA/RNA Shield™ ensures nucleic acid stability during sample storage/transport at ambient temperatures (4-25°C). The reagent effectively lyses cells and inactivates nucleases and infectious agents (virus).
The kit also features a specialized buffer system that facilitates complete viral
particle lysis for efficient RNA isolation from samples containing enteroviruses,
rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus, parvovirus (a ssDNA virus), etc. Viral RNA is bound to the column, washed and eluted.
The isolated high-quality viral RNA is ready for all downstream applications such as Next-Gen Sequencing, hybridization-based and RT/PCR detection.

Technical Specifications

Equipment	Microcentrifuge
Purity	RNA is ready for Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.
Sample Source	Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, and swab and fecal samples stored in DNA/RNA Shield
Size Range	50 nt to ~200 kb
Yield	10 µg RNA (binding capacity), ≥6 µl (elution volume)

Citations

Reviews

“The Quick-RNA Viral RNA kit performed much better than the RNeasy 96 kit for extracting RNA from dilute samples of virus supernatant. The kit is designed for small-volume elution to automatically concentrate the sample, and it’s designed for viral supernatants rather than cell lysates, so we didn’t need to deal with optimizing added carrier RNA. So in all, it was simpler, easier, and gave us a 10-fold improvement in our limit of detection.”

– S.B., Boehringer Ingelheim, Canada

Kit Components

Cat #	Name	Size
R1200-25	DNA/RNA Shield (2X Concentrate)	25 ml
R1200-125	DNA/RNA Shield (2X Concentrate)	125 ml
W1001-10	DNase/RNase-Free Water	10 ml
W1001-4	DNase/RNase-Free Water	4 ml
R1034-1-100	Viral RNA Buffer	100 ml
R1034-1-50	Viral RNA Buffer	50 ml
R1034-2-48	Viral RNA Wash Buffer (Concentrate)	48 ml
R1034-2-6	Viral RNA Wash Buffer (Concentrate)	6 ml
C1001-50	Collection Tubes	50 Pack
C1004-50	Zymo-Spin IC Columns	50 Pack

Quick-RNA Viral Kit

R1034 / R1035

Cat #	Name	Size
R1034	Quick-RNA Viral Kit	50 preps
R1035	Quick-RNA Viral Kit	200 preps

Documents

Protocol:

Datasheet:

SDS (MSDS):

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Frequently Asked Questions

Are your products FDA-cleared?

Zymo Research’s products are considered for research use only.

Because the virus (SARS-CoV-2) causing COVID-19 is new, there are no FDA-cleared or approved tests to diagnose or detect it. However, during public health emergencies the FDA is able to issue Emergency Use Authorizations (EUAs) which allows test manufacturers to distribute potentially life-saving technologies prior to clearance or approval after the performance characteristics have already been validated. You can find more information at www.fda.gov/emergency-preparedness-and-response/mcm-legal-regulatory-and-policy-framework/emergency-use-authorization

Zymo Research is actively working with the FDA to have an EUA approved for a SARS-CoV-2 detection test with its corresponding workflow. This workflow includes DNA/RNA Shield Collection Devices, Quick-DNA/RNA Viral MagBead Kit (R2140), and the Quick-SARS-CoV-2 rRT-PCR Test Kit.

Health organizations have validated Zymo Research’s sample collection and viral extraction kits in their workflows for clinical specimen collection and processing. For the adoption of Zymo products into EUA workflows, further validation on the product’s performance are required. Feel free to contact us, for additional information or assistance: customerserivce@biolinkk.com.

What viral extraction kits do you recommend?

We offer a variety of viral extraction kit formats for both DNA and RNA. For high-throughput, automatable options, we highly recommend our Quick-DNA/RNA Viral MagBead Kit. Our technical support team is standing by to assist with any product inquiries: customerserivce@biolinkk.com.

What automatable options do you provide for viral extraction?

For high-throughput, automatable options, we recommend our Quick-DNA/RNA Viral MagBead Kit. Scripts are available for Tecan, Hamilton, and KingFisher automated platforms. Please contact our automation specialists for further support: customerserivce@biolinkk.com.

Can you support our COVID-19 testing lab or project?

Yes. We have a dedicated team of specialists readily available to assist you with questions regarding product availability, product recommendations and regulatory requirements. Please email customerserivce@biolinkk.com.

Quick-DNA/RNA Viral MagBead Formats

	Input	Binding Capacity	Elution Volume	Equipment Needed	Instruction Manual
1 x 96 preps 4 x 96 preps	≤ 400 µl plasma, serum, urine, cell culture media, blood, saliva, cellular suspension, swab, fecal sample or ≤ 5 mg biopsy sample.	10 µg DNA/RNA per 20 µl MagBinding Beads	≥50 µl	Manual: Magnetic stand or separator Automated: Magnetic stand or separator Liquid handler or robotic sample processor

Input

Binding Capacity

Elution Volume

Equipment Needed

Instruction Manual

1 x 96 preps

4 x 96 preps

≤ 400 µl plasma, serum, urine, cell culture media, blood, saliva, cellular suspension, swab, fecal sample or ≤ 5 mg biopsy sample.

10 µg DNA/RNA per 20 µl MagBinding Beads

≥50 µl

Manual:
Magnetic stand or separator

Automated:
Magnetic stand or separator
Liquid handler or robotic sample processor

*Quick-DNA/RNA Viral MagBead kit is for research use only (RUO).