- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased: Complete RNA recovery without miRNA loss.
Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA.
The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.).
The entire procedure typically takes only 7 minutes.
|Compatibility||TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.|
|Sample Inactivation||TRI Reagent® (provided with R2051, R2053) inhibits RNase activity and inactivates viruses and other infectious agents.|
|Sample Source||Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).|
|Size Range||Total RNA ≥ 17 nt|
|Yield||50 µg RNA (binding capacity), ≥25 µl (elution volume)|
Q1: Is DNase I available for individual purchase?
Q2: How to store DNase-I following resuspension?
Q3: Is the kit compatible with samples stored in DNA/RNA Shield?
Q4: Is Direct-zol suitable for very small numbers of cells?
Q5: Is it possible to extract proteins with the Direct-zol RNA kits?
Q6: Is the DNase-I treatment necessary?
Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?
Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Q10: Which phenol-based reagents are compatible with Direct-zol?
Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
Q13: I ran out of RNA Wash Buffer. Can I use something else?
“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”
– Adina B. (University of Guelph)
“This kit is amazing, I’ve got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“
– R.K. CSU
“Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”
– H.Z. (Harvard Medical School)
“Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? Just one column and two buffers, I love it.”
– Arjan V. (Indiana University)
“Simple protocol and yielded good quality of RNA. Only one kit working for all type of tissue, cell and especially biological fluids.”
– Mohan K. (University of Illinois, Chicago)
“The Direct-zol RNA MiniPrep showed the highested recovery [of miRNA] from both cell culture and frozen post-mortem human brain tissue when compared to miRNeasy, mirVana and RNeasy Plus.”
– O.E. (University of Southern California)
“The yield and the purity of the RNA is something out of this world, about at least 50% more RNA and purity not less than 1.95, we never got [that] before with other vendors such Invitrogen, Qiagen, etc.”
– Ehab S. (University of Iowa)
“Before I discovered this kit, I was isolating RNA the old school way with chloroform and it would take half the day to finish the protocol. The Direct-zol RNA Miniprep kit is AWESOME! It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench.”
– A. Newhart (The Wistar Institute)
Direct-zol RNA Miniprep Kits