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- High-throughput purification of high quality, inhibitor-free DNA from any sample including feces, soil, water, biofilms, swabs, saliva, and body fluids.
- The ZymoBIOMICS innovative lysis system enables efficient and unbiased lysis of microbes including Gram-positive/negative bacteria, fungi, protozoans, algae, etc.
- The automation friendly workflow enables nearly any sample to be processed in as little as 90 minutes for 96 preps.
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. |
|---|---|
| Elution Volume | ≥ 37.5 µl DNA Elution Buffer or DNase free water |
| Equipment | Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block |
| Processing Volume | Fecal ≤100 mg, Soil ≤ 200 mg, Cells ≤ 20 mg (Approximately equal to 2×108 bacterial, 2×107 yeast, or 2×106 mammalian cells) |
| Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. |
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
| Type | Total DNA |
| Yield | Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum) |
| Supplemental Info |
FAQs
Yes, we currently have scripts for Hamilton and KingFisher, as well as support for Tecan on some systems. Please contact us for scripts specific to your setup.
Yes, our protocol includes an “automation guide” for users to script the protocol themselves and our technical support staff are available for any automation help, troubleshooting, and advice.Â
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Reviews
Based on 4 reviews
Trusted reviews provided by
Timothy Butler
Trace Genomics
Mar 29 2022
Great results, easy to use
This kit is easy to use and effective for microbial DNA purification. Its process can be streamlined for use with automation also.
Application Area:Â Molecular Biology
Luke Thompson
NOAA / Mississippi State University
Jan 26 2022
Good kit for doing 96-well mag-bead DNA extractions, eg with a KingFisher robot
This has been a great product for extracting DNA from filters. The DNA just needs to be removed from the filters by vortexing or bead-beating before adding the the 96-well plate. Depending on the protocol variation, some reagents are consumed faster than others, but it has been possible to order additional reagents from Zymo.
Application Area:Â DNA extraction using magnetic beads
Timothy Butler
Trace Genomics
Jul 28 2021
Great results, high throughput.
Love this kit. It is easy to use and fast. Good for high throughput labs.
Application Area:Â Soil DNA extraction/purification
Alvaro Munoz
Scripps Institution of Oceanography, UCSD
Mar 16 2021
Amazing kits and supplies, Zymo also goes beyond regarding their customer service.
Zymo kits and consumables are always a good option and the fact that they allow you to test their products on your application for free is amazing. I have not seen any product be considerably outperformed by any of the other brands that supply similar products. Their customer service is consistently outstanding with fast response timing and always willing to help you find the best product for your application. I always recommend getting in contact with their technical service to discuss any questions you may have. There are many times when they have pointed me to an amazing product they offer but that I had no idea was available. It is extremely valuable that they offer most of the components of their kits sold separately so you can purchase exactly what you need and not spend your funds on supplies you did not need.
Application Area:Â Molecular biology and genome seqeuncing
Kit Components
| Cat # | Name | Size |
|---|---|---|
| D4302-3-100 | ZymoBIOMICS MagWash 1 | 100 ml |
| D4300-1-150 | ZymoBIOMICS Lysis Solution | 150 ml |
| D4302-4-200 | ZymoBIOMICS MagWash 2 | 200 ml |
| D4302-5-50 | ZymoBIOMICS DNase/RNase Free Water | 50 ml |
| D4302-6-12 | ZymoBIOMICS MagBinding Beads | 12 ml |
| S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes |
| S6002-96-3 | ZR-96 BashingBead Lysis Rack | 0.5 mm & 0.1 mm |
ZymoBIOMICS 96 MagBead DNA Kit
| Cat # | Name | Size |
|---|---|---|
| D4302 | ZymoBIOMICS 96 MagBead DNA Kit (Lysis Rack) | 2 x 96 Preps |
| D4306 | ZymoBIOMICS 96 MagBead DNA Kit (No Lysis Matrix) | 2 x 96 Preps |
| D4308 | ZymoBIOMICS 96 MagBead DNA Kit (Lysis Tubes) | 2 x 96 Preps |
| D4302-E | ZymoBIOMICS 96 MagBead DNA Kit – Dx (Lysis Rack) | 2 x 96 Preps |
| D4306-E | ZymoBIOMICS 96 MagBead DNA Kit – Dx (No Lysis Matrix) | 2 x 96 Preps |
| D4308-E | ZymoBIOMICS 96 MagBead DNA Kit – Dx (Lysis Tubes) | 2 x 96 Preps |