KEY FEATURES:• Amine-reactive; has a spacer with arm length of 22.4 Å which helps to minimize steric hindrance to biotin labeling• More water-soluble than the corresponding NHS compound without the sulfo group• Membrane-impermeable• Forms permanent amide bonds and the spacer arm cannot be cleavedAPPLICATIONS:• Sulfo-NHS-LC-Biotin is ideal for biotinylating antibody or protein that can then be used for protein detection, purification or immobilization • Sulfo-NHS-LC-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent
|SKU-Size||2323-set (Size: 1 set (10 x 1 mg))
2323-50 (Size: 50 mg)
2323-100 (Size: 100 mg)
|Alternate Name||Sulfosuccinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate; Biotin disulfide N-Hydroxysulfosuccinimide ester|
|Appearance||White to off-white solid|
|Purity||≥80% by NMR|
|Solubility||H₂O (~ 6 mg/ml)|
|Handling||Protect from moisture|
|Storage Conditions||4 ̊C|
|Shipping Conditions||Gel Pack|
|USAGE||For Research Use Only! Not For Use in Humans.|
When a protein is biotinylated by Sulfo-NHS-LC-Biotin, which part of this molecule attached to protein?
Sulfo-NHS-LC-biotin (Sulfosuccinimidyl 6-(biotinamido) Hexanoate) is an amine-reactive biotinylation agent. It covalently binds to the amine groups in proteins and other molecules through its NHS ester forming an amide linkage.
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