Description
KEY FEATURES:• Amine-reactive; has a spacer with arm length of 22.4 Å which helps to minimize steric hindrance to biotin labeling• More water-soluble than the corresponding NHS compound without the sulfo group• Membrane-impermeable• Forms permanent amide bonds and the spacer arm cannot be cleavedAPPLICATIONS:• Sulfo-NHS-LC-Biotin is ideal for biotinylating antibody or protein that can then be used for protein detection, purification or immobilization • Sulfo-NHS-LC-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent
Datasheet
| SKU-Size | 2326-set (Size: 1 set (10 x 1 mg)) 2326-50 (Size: 50 mg) 2326-100 (Size: 100 mg) |
|---|---|
| Alternate Name | Sulfosuccinimidyl 6-(biotinamido) Hexanoate; Biotinamidohexanoic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt |
| Appearance | White to Off-white Solid |
| CAS # | 127062-22-0 |
| Molecular Formula | C₁₄H₁₈O₈N₃S₂Na |
| Molecular Weight | 556.59 |
| Purity | ≥90% by NMR |
| Solubility | H₂O (~ 20 mg/ml) |
| SMILES | O=C([MH]1)NC2C1CSC2CCCCC(NCCCCCC(ON3C(C(S(=O)(O[Na])=O)CC3=O)=O)=O)=O |
| InChi | InChI=1S/C20H30N4O9S2.Na/c25-15(7-4-3-6-13-18-12(11-34-13)22-20(29)23-18)21-9-5-1-2-8-17(27)33-24-16(26)10-14(19(24)28)35(30,31)32;/h12-14,18H,1-11H2,(H,21,25)(H2,22,23,29)(H,30,31,32); |
| InChi Key | RDSJVNGBJUJRRW-UHFFFAOYSA-N |
| PubChem CID | 16219001 |
| MDL Number | MFCD00078534 |
| Handling | Protect from moisture |
| Storage Conditions | -20 ̊C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
FAQ
When a protein is biotinylated by Sulfo-NHS-LC-Biotin, which part of this molecule attached to protein?
Sulfo-NHS-LC-biotin (Sulfosuccinimidyl 6-(biotinamido) Hexanoate) is an amine-reactive biotinylation agent. It covalently binds to the amine groups in proteins and other molecules through its NHS ester forming an amide linkage.
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