KEY FEATURES:• Sulfo-LC-SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed• Amine-reactive; the "long chain" Sulfo-LC-SPDP crosslinking reagent has a 15.7 Å spacer arm• More water-soluble than the corresponding LC-SPDP• Membrane-impermeable which enables crosslinking on cell surface• The cleavable spacer arm enables separation of cross-linked productsAPPLICATIONS:• Reversible crosslinking of proteins• N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains• Sulfo-LC-SPDP crosslinking molecule pyridinyldisulfide reacts with sulhydryls to yield a reversible disulfide bond.
|SKU-Size||2325-50 (Size: 50 mg)
2325-50 (Size: 50 mg)
|Alternate Name||Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexanoate|
|Appearance||White to Off-white Solid|
|Purity||≥90% by NMR|
|Solubility||H₂O (~ 6 mg/ml)|
|Handling||Protect from moisture|
|Storage Conditions||-20 ̊C|
|Shipping Conditions||Gel Pack|
|USAGE||For Research Use Only! Not For Use in Humans.|
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