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SPDP Crosslinker: A cleavable heterobifunctional crosslinker
Datasheet (PDF) | Safety Data Sheets (MSDS)(PDF)
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Description
KEY FEATURES:• SPDP contains a dithiopyridyl group which is reactive towards cysteines (-SH groups) and an NHS group, which is useful when targeting primary amines (-NH2 groups)• SPDP releases a byproduct, pyridine-2-thione after reacting with free sulfhydryl groups. By measuring the release of this byproduct at 343 nm, the reaction can be easily followed• Contains a disulfide linkage in its spacer arm with a length of 6.8 Å• The spacer is cleavable with reducing agent, such as DTT, TCEP, etc.APPLICATIONS:• Reversible crosslinking of proteins• N-hydroxysulfosuccinimide (Sulfo-NHS) ester that reacts with primary amines, as found in lysine side chains• Useful for adding sulfhydryl groups to proteins by attachment to lysine residues• Useful for preparation of enzyme immunoconjugates and hapten carrier molecule conjugates
Datasheet
| SKU-Size | 2328-50 (Size: 50 mg) 2328-50 (Size: 50 mg) |
|---|---|
| Alternate Name | N-succinimidyl-3-(2-pyridyldithio)propionate; 3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester; 3-(2-Pyridyldithio)propionic acid NHS ester |
| Appearance | White to Off-white Solid |
| CAS # | 68181-17-9 |
| Molecular Formula | C₁₂H₁₂N₂O₁₀ |
| Molecular Weight | 312.36 |
| Purity | ≥90% by NMR |
| Solubility | DMSO or DMF |
| SMILES | C1CC(=O)N(C1=O)OC(=O)CCSSC2=CC=CC=N2 |
| InChi | InChI=1S/C12H12N2O4S2/c15-10-4-5-11(16)14(10)18-12(17)6-8-19-20-9-3-1-2-7-13-9/h1-3,7H,4-6,8H2 |
| InChi Key | JWDFQMWEFLOOED-UHFFFAOYSA-N |
| PubChem CID | 100682 |
| MDL Number | MFCD00009636 |
| Handling | Protect from moisture |
| Storage Conditions | -20 ̊C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
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