RNAzol® RT Column Kit
Full Protocol (PDF) | SDS (PDF)
View All Isolation Kits
Catalog# RC 290
Price Not Available
RNAzol® RT is the most effective reagent for isolation of total RNA and small RNA from samples of human, animal, plant, bacterial and viral origin. This patented reagent(1) provides higher yield and quality of isolated RNA than previous reagents based on the single-step method. RNAzol® RT isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment.(2)
• No chloroform-induced phase separation is necessary to obtain pure RNA. Just add water to remove DNA, proteins, polysaccharides and other contaminants.
• The isolation procedure can be completed in less than one hour and is performed at room temperature, including all centrifugation steps.
• RNAzol® RT isolates total RNA, or large RNA and small RNA in separate fractions. The large RNA fraction contains rRNA and mRNA. The small RNA fraction contains tRNA, small RNA and microRNA down to 10 bases.
• The isolated RNA is ready for RT-PCR, qRT-PCR, microarrays, poly A+ selection, northern blotting, RNase protection assay and other molecular biology applications.
• Due to the removal of impurities, the RNA pellets are smaller and solubilize more easily than pellets obtained from previous single-step reagents.
• In addition, RNAzol® RT allows for the simultaneous isolation of RNA and DNA.
RNAzol® RT is used to isolate RNA from tissues, cells, liquid samples or blood. One milliliter is sufficient to process up to 100 mg tissue yielding 50 – 700 μg of large RNA (>200 bases) and 8 – 120μg of small RNA (200 – 10 bases).
Catalog # RC 290: 50 ml RNAzol® RT, 50 Columns with Collection Tubes, 50 Wash Tubes, 50 Elution Tubes, 10 ml RNase-free Water
Catalog # RC 290T: Trial size available-10 isolations
1. US patents 7,794,932 and 8,367,817. International patents.
Full Protocol (PDF)
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact Tech Support
High Quality Guaranteed Product
Our products such as Elisa, Antibodies, Proteins, Peptides and sequencing kits are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.