Highlights
- NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
- Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
- Maximum Recovery: Recover >90% and elute in as little as 6 ÎĽl.
Description
The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
Technical Specifications
| Equipment Required | Microcentrifuge |
|---|---|
| Format | Spin-Column |
| Purity | RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8. |
| Sample Source | DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction |
| Size Range | Total RNA ≥ 17 nt |
| Yield | 10 µg RNA (binding capacity), ≥ 6 µl (elution volume) |
Product Faq
Yes. The kit efficiently recovers all types of nucleic acids.
Carrier RNA can be added with no detrimental effects. The RCC is highly efficient without the need for carrier RNA, and no significant improvement in recovery has been observed with carrier RNA.
Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin
Product Tolerance Reference: – ≤5% Triton X-100 – ≤5% Tween-20 – ≤5% Sarkosyl – ≤0.1% SDS – ≤90% formamide – Sucrose samples should be diluted/titrated down 10- to 100- fold.
Yes, follow respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post clean-up, then re-purify.
If the downstream application requires DNA-free RNA, we would recommend performing in-column or in-tube DNase I treatment.
Citation
| 7681086 | Published 5 Jan 2021 |
‘We then cleaned up the cfRNAs using RNA Clean and Concentrator-5 kit (Zymo), and eluted them with 7 µL elution buffer.’
| Cell Rep Med | Published 22 Dec 2020 |
’10 min and then at 80°C for 10 min to inactivate the enzymes. … To remove the RNA template, 1 ÎĽL of RNase H (Invitrogen) was added to each reaction mixture, followed by incubation at 37°C for 20 min. To remove unused cDNA primer, samples were purified using an RNA clean and concentrator kit (Zymo Research CatR1017). cDNA was eluted in RNase/DNase-free water and the entire sample was used for PCR amplification. cDNA was amplified using a hemi-nested PCR.. We used Phusion DNA polymerase (ThermoFisher Cat#F530L) for both rounds of PCR.’
| Biomedicines | Published 18 Dec 2020 |
‘Nanostring Gene Expression Assay and Target AnalysisFor the nanostring gene expression assay sampling, sorted cells from 4 to 5 animals were pooled.. The RNA was isolated from cells by using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) with additional purification and concentration with RNA Clean & Concentrator (Zymo Research Irvine, CA, USA).. Nanostring technology allows one to receive a signal from a sample, not relative to another—for example, with array CGH (also put on chips, but external control is..
reviews
“It isolates small RNAs in the same go, unlike MinElute columns which leaves out RNAs smaller than 200nt.”
– Anupma C.
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“The Zymo-Spin IC column is convenient.”
– Lihua D. (National Institutes of Health)
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“Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do.”
– Caroline S. (Cornell University)
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“Great for purifying small amounts of RNA. I use a bioanalyzer after and I can see the difference from before, cleaner bands and the concentration increases with a smaller amount of elutant.”
– Catherine A. (USAMRIID)
“The product is very easy to use, very quick protocol for an excellent RNA clean up. It also purifies small RNA which is not the case of all RNA clean up columns.”
– Agnes N. (Biofidal)
“Zymo RNA Clean & Concentrator is the best product for CRISPR applications. We have tried several other products including precipitation methods, Zymo product gave us the best results and yield.”
– Gaurav V. (OMRF)
“Great result. Everybody should try this product. I’ve never seen such purity of the RNA before. This product works excellent and easy to use and affordable.”
– Para S. (University of Louisville)
“I used this as a clean-up step after guanidinium thiocyanate-phenol-chloroform extraction from homogenized tissues. I found the process very simple, and adding the kit on did seem to significantly improve the quality of the RNA pool relative to the original phenol-chloroform extraction.”
– Emily G. (University of Wisconsin-Madison)
“It’s [an] excellent kit to work with tissues with high poly-phenols and acids.”
– Arun D. (University of Missouri)
“Great yield from a tiny amount of genomic RNA.”
– Andrew H. (University of British Columbia)
kit components
| Cat # | Name | Size |
|---|---|---|
| W1001-4 | DNase/RNase-Free Water | 4 ml |
| W1001-10 | DNase/RNase-Free Water | 10 ml |
| C1004-50 | Zymo-Spin IC Columns | 50 Pack |
| R1060-2-25 | RNA Prep Buffer | 25 ml |
| R1060-2-100 | RNA Prep Buffer | 100 ml |
| C1001-50 | Collection Tubes | 50 Pack |
| R1003-3-12 | RNA Wash Buffer | 12 ml |
| R1003-3-24 | RNA Wash Buffer | 24 ml |
| R1013-2-100 | RNA Binding Buffer | 100 ml |
| R1013-2-25 | RNA Binding Buffer | 25 ml |
| E1010-1-4 | DNA Digestion Buffer | 4 mL |
RNA Clean & Concentrator-5
| Cat# | Name | Size |
|---|---|---|
| R1013 | RNA Clean & Concentrator-5 (DNase Included) | 50 Preps |
| R1014 | RNA Clean & Concentrator-5 (DNase Included) | 200 Preps |
| R1015 | RNA Clean & Concentrator-5 (DNase not Included) | 50 Preps |
| R1016 | RNA Clean & Concentrator-5 (DNase not Included) | 200 Preps |
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