NADH disodium salt, trihydrate, reduced

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Cat.#: NB0642

Specifications:

 Size: 1g
 CAS: [606-68-8]
 Grade:  Reagent
 DG: No
 Storage:  (-15 to -20)C
 Sterile: No
 Catalog Number  Size  Price (INR)
NB0642 1g
5g
5106.00
20976.00


Product Details


Product Description:    NADH disodium salt, trihydrate, reduced: β-NADH, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. β-NAD+ is a carrier for hydride ion, forming β-NADH. The hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as, malic dehydrogenase and lactic dehydrogenase. These enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to β-NAD+, forming the reduced product, β-NADH. Unlike β-NAD+ which has no absorbance at 340 nm, β-NADH absorbs at 340 nm. The increase in absorbance (with the formation of β-NADH) or the decrease in absorbance (with the formation β-NAD+) is the basis for measurement of activity of many enzymes at 340 nm.Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NADH as a cofactor and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. β-NADH is fluorescent whereas β-NAD+ is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10- M. Discussion of optimizing the fluorescence intensity and identification of interfering substances has been reported. β-NADH should be stored desiccated and protected from light.


Total Product Size:   250mg/1g/5g


Individual Container Size:   1g/5g


Number of Containers:   1


Shipping Conditions:    ICE


UNSPSC Code:     41106305


UNSPSC Category:     Nucleotides


Extra Info.:     Refrigeration Requirements: Freezer          


Documents


Certificate of Analysis (COA):

COA

Material Safety Data Sheets (SDS):

MSDS

Datasheet:

DATASHEET


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