Mitochondrial DNA Purification Kit
Datasheet (PDF) | Safety Data Sheets (MSDS)(PDF)
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Description
Mitochondria are essential double-membrane bound organelles that support aerobic respiration and most of the cellular ATP production in all nucleated mammalian cells. To date, hundreds of pathogenic mitochondrial DNA mutations have been identified to result in disease phenotypes such as cancer, ataxia, and diabetes. As a result, isolation of high quality mitochondrial DNA from mammalian cells and tissues in sufficient quantities is important for many basic research and clinical applications. BioVision’s Mitochondrial DNA Isolation Kit facilitates purification of quality mitochondrial DNA from mammalian cultured cells or tissue. This kit utilizes a density separation step to isolate mitochondria from the rest of the cellular organelles, followed by an enzymatic reaction to release DNA from the mitochondria. Upon release, mitochondrial DNA is further purified from nuclear DNA contamination and adsorbed onto a silica spin-column under chaotropic conditions, eliminating the use of toxic organic compounds or solvents. Mitochondrial DNA purified by this kit is suitable for various downstream molecular biology applications such as PCR, cloning, DNA hybridization, Southern Blotting, sequencing, genotyping, enzymatic analysis, and more.
Datasheet
| #Cat + Size | K389-25 (Size: 25 Purifications) |
| Kit Summary | • Species reactivity- Mammalian • PCR, cloning, DNA hybridization, southern blotting, genotyping, restriction enzyme digest analysis, sequencing, etc. |
| Species Reactivity | Mammalian |
| Applications | PCR, cloning, DNA hybridization, southern blotting, genotyping, restriction enzyme digest analysis, sequencing, etc. |
| Features & Benefits | • Simple procedure; takes less than 2 hours • Fast and convenient • The kit provides unique formulations of buffers, reagents anc columns for isolating a highly enriched mitochondrial fraction. This kit provides genomic-free DNA samples |
| Kit Components | • Buffer H [Homogenization Buffer] • Buffer R [Re-suspension Buffer] • Enzyme Mix • RNAse A • Buffer L [Lysis Buffer] • Buffer N [Neutralization Buffer •Buffer W [Wash Buffer] • Buffer E [Elution Buffer] • Spin Columns/Collection Tubes |
| Storage Conditions | -20°C/RT |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
FAQ
Can you describe the differences between K389-25 and K280-50?
K389 Mitochondrial DNA Isolation Kit facilitates purification of high quality mitochondrial DNA from mammalian cultured cells or tissue. A density gradient separation method is utilized to isolate mitochondria from the rest of the cellular organelles. This is followed by an enzymatic reaction to release DNA from the mitochondria. Upon release, mitochondrial DNA is further purified from nuclear DNA contamination and adsorbed onto a silica spin-column under chaotropic conditions, eliminating the use of toxic organic compounds or solvents. K280 is not based on density gradient separation of the mitochondria, but it is a reagent based methodology. For K280, ~5-20 µg of mtDNA can be isolated based on the starting material. For K389, ~2 -20 µg of DNA can be obtained based on the starting number of cells/tissues as specified in the data sheet.
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