Home >Protein Sample Preparation and Cell Fractionation >Minute™ High Fidelity ER Isolation Kit for Liver Tissue (20 Preps)
Minute™ High Fidelity ER Isolation Kit for Liver Tissue (20 Preps)
Manual & Protocol (PDF) | Material Safety Data Sheets (MSDS)(PDF)
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Description
Endoplasmic reticulum (ER) is a major membranous structure that functionally connects nuclear membrane and plasma membrane. Liver is an ER-rich organ and has been used for isolation of ER for decades. Traditional methods for isolating ER are based on density-gradient ultracentrifugation. The protocol requires a large amount of starting material and the methods are tedious and time-consuming and contain significant cross-contamination. Currently, all commercial kits for ER isolation are based on methods developed in the 1970s. Unlike any other ER isolation kit on the market, this kit employs a patented spin-column-based technology that is simple and rapid and requires only a small amount of tissue. This kit can differentially precipitate native ER (mainly rough ER) from frozen liver tissues without using a Dounce homogenizer or ultracentrifugation. The whole protocol can be done in about 2 hours.
Kit Includes
| Items | Quantity |
| Buffer A | 20 ml |
| Buffer B | 4 ml |
| Buffer C | 4 ml |
| Filter Cartridges | 20 Units |
| Collection Tubes | 20 Units |
| Plastic Rods | 2 Units |
References
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