Minute™ Golgi Apparatus Enrichment Kit (20 Preps)


Minute™ Golgi Apparatus Enrichment Kit (20 Preps)


Manual & Protocol (PDF)   |   Material Safety Data Sheets (MSDS)(PDF)

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Catalog# GO-037

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Description




Golgi apparatus, also called Golgi complex or Golgi body, is made up of a series of flattened stacked pouches (cisternae). Golgi apparatus is a vital organelle in eukaryotic cells and is responsible for transporting, modifying, and packaging protein and lipids into vesicles for delivery to targeted locations. The quantity and distribution of Golgi in different cell and tissue types vary significantly. The ability to obtain highly-enriched Golgi fraction is an important first step for studying its function and interaction with other organelles. Traditional methods for isolating Golgi apparatus are based on density gradient ultracentrifugation. The protocol requires a large amount of starting material and the methods are tedious and time-consuming. Unlike any other Golgi isolation kit, this kit uses a patented spin-column-based technology that is simple, rapid and requires only a small amount of starting material. This kit can preferentially enrich native Golgi by precipitation without using a Dounce homogenizer and ultracentrifugation. Two sub-Golgi fractions can be obtained: Golgi apparatus and secretory vesicles of Golgi.












Kit Includes




Items Quantity
Buffer A 20 ml
Buffer B 8 ml
Buffer C 2 ml
Buffer D 2 ml
Filter Cartridges 20 Units
Collection Tubes 20 Units
Plastic Rods 2 Units




References




1. Tan, X., Shi, L., Banerjee, P., Liu, X., Guo, H. F., Yu, J., ... & Creighton, C. J. (2020). A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma. The Journal of Clinical Investigation.

2. Zhu, Y., Shao, F., Yan, W., Xu, Q., & Sun, Y. (2020). Inhibition of SHP2 ameliorates psoriasis by decreasing TLR7 endosome localization. medRxiv.

3. Lita, A., Pliss, A., Kuzmin, A., Yamasaki, T., Zhang, L., Dowdy, T., ... & Larion, M. (2021). IDH1 mutations induce organelle defects via dysregulated phospholipids. Nature Communications, 12(1), 1-16.

4. Tan, X., Banerjee, P., Shi, L., Xiao, G. Y., Rodriguez, B. L., Grzeskowiak, C. L., ... & Kurie, J. M. (2021). p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi. Science Advances, 7(25), eabf4885.






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