Minute™ Cytoplasmic & Nuclear Extraction Kits for Cells (50 Preps)


Minute™ Cytoplasmic & Nuclear Extraction Kits for Cells (50 Preps)


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Catalog# SC-003

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Description




Minute™ Cytoplasmic and Nuclear Extraction Kit for Cells is composed of optimized cytoplasmic extraction buffer, nuclear protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to quickly separate native cytosol and nuclear proteins from mammalian cells and protoplasts of plant, bacteria, yeast and fungus. Separation of cytoplasmic and nuclear proteins can be accomplished in less than 15 minutes using the protein extraction filter cartridges.












Kit Includes




Items Quantity
Cytoplasmic Extraction Buffer 25 ml
Nuclear Extraction Buffer 25 ml
Protein Extraction Filter Cartridges 50 units
Collection Tubes with Caps 50 units




References




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3. Hou, N., et al. (2014). Vitamin A deficiency impairs spatial learning and memory: The mechanism of abnormal CBP-dependent histone acetylation regulated by retinoic acid receptor alpha. Mol Neurobiol. DOI 10.1007/s12035-014-8741-6.

4. You, Y., et al. (2014). Latexin sensitizes leukemogenic cells to gamma-irradiation-induced cell-cycle arrest and cell death through Rps3. Cell Death and Disease. (2014)5, e1493; doi: 10.1038/cddis. 2014.443.

5. Tanaka M et al. (2016). Ex vivo effects of naphthoquinones allergen-sensitized mononuclear cells in mice. International Journal of Immunopathology and Pharmacology. DOI: 10.1177/0394632016632221

6. Liu W. et al. (2016) Electroacupunctre improves motor impairment via inhibition of microglia-mediated neuroinflammation in the sensorimotor cortex after ischemic stroke. Life Sciences. doi:10.1016/j.lfs.2016.01.045

7. Xu, Yang, et al. (2016), RIP3 induces ischemic neuronal DNA degradation and programmed necrosis in rat via AIF." Scientific Reports 6:29362.

8. Shi, Le, et al. (2016). "Evodiamine exerts anti-tumor effects against hepatocellular carcinoma through inhibiting β-catenin-mediated angiogenesis." Tumor Biology: 1-13.9

9. Matsuoka, Y., Nakayama, H., Yoshida, R., Hirosue, A., Nagata, M., Tanaka, T., ... & Shinriki, S. (2016). IL-6 controls resistance to radiation by suppressing oxidative stress via the Nrf2-antioxidant pathway in oral squamous cell carcinoma. British Journal of Cancer.

10. Ueda, K., Masuda, A., Fukuda, M., Tanaka, S., Hosokawa, M., & Iwakawa, S. (2017). Monophosphorylation by deoxycytidine kinase affects apparent cellular uptake of decitabine in HCT116 colon cancer cells. Drug Metabolism and Pharmacokinetics.

11. Kawaguchi, R., Hirata, N., Tokinaga, Y., Hayashi, S., Inoue, S., Watanabe, N., & Yamakage, M. (2017). Nitrite administration improves sepsis-induced myocardial and mitochondrial dysfunction by modulating stress signal responses. Journal of Anesthesia, 31(6), 885-894.

12. Kong, P., Yu, Y., Wang, L., Dou, Y. Q., Zhang, X. H., Cui, Y., ... & Cui, W. (2019). circ-Sirt1 controls NF-κB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells. Nucleic Acids Research.

13. Kang, Y., Li, Y., Zhang, T., Chi, Y., & Liu, M. Effects of transcription factor EB on oxidative stress and apoptosis induced by high glucose in podocytes. International Journal of Molecular Medicine. 44(2), 447-456.

14. Yuan, H. X., Chen, C. Y., Li, Y. Q., Ning, D. S., Li, Y., Chen, Y. T., ... & Jian, Y. P. (2020). Circulating extracellular vesicles from patients with valvular heart disease induce neutrophil chemotaxis via FOXO3a and the inhibiting role of dexmedetomidine. American Journal of Physiology-Endocrinology adoi.org/10.1007/s13402-020-00540-6nd Metabolism.

15. Dang, H., Chen, L., Tang, P., Cai, X., Zhang, W., Zhang, R., ... & Tang, H. (2020). LINC01419 promotes cell proliferation and metastasis in hepatocellular carcinoma by enhancing NDRG1 promoter activity. Cellular Oncology, 1-17.






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