Minute™ Cell Suspension Isolation Kit from Fresh/Frozen Tissues (50 Preps)

Minute™ Cell Suspension Isolation Kit from Fresh/Frozen Tissues (50 Preps)

Manual & Protocol (PDF)   |   Material Safety Data Sheets (MSDS)(PDF)

View All Related Products

Catalog# CS-031


For orders and inquiries

Contact Us


Obtaining quality cell suspension from fresh and/or frozen tissues is an important first step for subsequent analysis such as flow cytometry analysis (FACS) and nucleic acid purification. Cell suspensions can be isolated from tissues by one or a combination of the following three mechanisms: chemical tissue dissociation, enzymatic digestion and physical separation. Many methods are tedious and time consuming. Most importantly, cells in the tissue, especially frozen tissue, are usually damaged and show low integrity and viability. It is very difficult to obtain high viability cell suspension from frozen tissues with high connective tissue contents such as liver, kidney and brain tissues. We have developed a simple, rapid, efficient, and instrument-free method for isolating cell suspension from fresh or frozen tissues using a combination of chemical tissue dissociation and physical separation mechanisms. The protocol can be finished in about 20 min with higher cell integrity and viability.

Kit Includes

Items Quantity
Tissue Dissociation Buffer 15 ml
1.5 ml Microfuge Tube 50 Units
Pestles for 1.5 ml Tube 2 Units


1. Zhang, X., Zhang, X., Li, X., Ma, J., Xie, D., & Jiang, N. (2020). Dishevelled-1 regulated apoptosis through NF-κB in Cerebral Ischemia/Reperfusion Injury in Rats. Neuroscience Letters, 134862.

2. Xu, H., Liu, L., Cong, M., Liu, T., Sun, S., Ma, H., ... & Wang, P. (2020). EGF neutralization antibodies attenuate liver fibrosis by inhibiting myofibroblast proliferation in bile duct ligation mice. Histochemistry and Cell Biology, 1-10.

Post Reviews

Leave a Reply

Your email address will not be published. Required fields are marked *

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact Tech Support

High Quality Guaranteed Product

Our products such as Elisa, Antibodies, Proteins, Peptides and sequencing kits are covered by Biolinkk quality warranty and will work as described in datasheet, a free replacement or money back is guaranteed if does not perform according to datasheet.

Learn More